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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 4: 751 - 759

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.04.004

Efficacy of Lushi Runzao decoction (路氏润燥汤) on ameliorating Sjogren's syndrome: a network pharmacology and experimental verification-based study

  • Fengtao PANG ,
  • Kesong LI ,
  • Yi ZHANG ,
  • Xiaopo TANG ,
  • Xinyao ZHOU
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  • Department of Rheumatology, Guang’anmen Hospital, Chinese Academy of Chinese Medical Sciences, Beijing 100053, China

Received date: 2022-02-22

  Accepted date: 2022-07-29

  Online published: 2023-04-04

Supported by

National Natural Science Foundation of China: Based on the Intestinal flora/SCFAs/FFA2 Approach to Explore the Mechanism of Lushi Runzao Decoction in Regulating Th17/Treg Immune Balance to treat Sjogren's Syndrome(82104837)

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Abstract

OBJECTIVE: To investigate the possible mechanism underlying the effect of the Lushi Runzao decoction (路氏润燥汤) on Sjogren’s syndrome using network pharmacology and to verify the mechanisms via animal experiments.

METHODS: Available biological data on each drug in the Lushi Runzao decoction were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, and the target proteins of Sjogren’s syndrome were retrieved from the GeneCards database. Information regarding Sjogren’s syndrome and the targets of the drugs were compared to obtain overlapping elements. This information was imported into the STRING platform to obtain a protein-protein interaction network diagram, following which a “component-target” network diagram was constructed using screened drug components and target information via Cytoscape software. The database for annotation, visualization, and integrated discovery was used for Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways analyses. Pathway information predicted by network pharmacology was verified using animal experiments.

RESULTS: The Lushi Runzao decoction ameliorated Sjogren’s syndrome mainly by influencing tumor necrosis factor as well as certain cytokines and chemokines. The decoction also influenced the interleukin-17 and advanced glycosylation end products (AGE)-receptor for AGE signaling pathways.

CONCLUSION: The Lushi Runzao decoction ameliorates Sjogren’s syndrome via multiple targets and multiple signaling pathways. Network pharmacology is useful for making a comprehensive prediction regarding the efficacy of the Lushi Runzao decoction, and this information may be helpful in clinical research.

Key words： immune system diseases; tumor necrosis factors; network pharmacology; Lushi Runzao decoction; experimental verification

Cite this article

Fengtao PANG , Kesong LI , Yi ZHANG , Xiaopo TANG , Xinyao ZHOU . Efficacy of Lushi Runzao decoction (路氏润燥汤) on ameliorating Sjogren's syndrome: a network pharmacology and experimental verification-based study[J]. Journal of Traditional Chinese Medicine, 2023 , 43(4) : 751 -759 . DOI: 10.19852/j.cnki.jtcm.2023.04.004

References

1. Nocturne G. Actualités dans le syndrome de Sj?gren primitif: aspects cliniques et thérapeutiques. La Revue de Médecine Interne 2019; 40: 433-9.
2. Ramos-Casals M, Brito-Zeron P, Siso-Almirall A, Bosch X. Primary Sjogren syndrome. BMJ 2012; 344: e3821.
3. Zhao J. Comparison of efficacy and safety between iguratimod and hydroxychloroquine in treating Sjogren’s syndrome. Zhong Guo Yao Wu Yu Lin Chuang 2020; 20: 3627-9.
4. Jiang Q. Lu Zhizheng’s academic thought inheritance and clinical application of regulating spleen and stomach to treat rheumatism. Beijing: Chinese Academy of Traditional Chinese Medicine, 2012: 12-8.
5. Deng J, Yu Z, Li X, et al. The combination of acupuncture and medicine in treating primary Sjogren’s syndrome dry mouth for 55 cases. Zhong Guo Zhong Yi Yao Xian Dai Yuan Cheng Jiao Yu 2017; 15: 40-2.
6. Zhang H, Jiang Q, Wang Z, et al. Observation on the therapeutic effect of Lushi Runzao decoction on dry eyes of primary Sjogren’s syndrome for 3 months. Shi Jie Zhong Xi Yi Jie He Za Zhi 2013; 8: 734-6.
7. Zhang H, Jiang Q, Huang Y, et al. Comparative study on the curative effect of Lushi Runzao decoction on dry mouth symptoms of primary Sjogren’s syndrome. Shi Jie Zhong Xi Yi Jie He Za Zhi 2013; 8: 578-81.
8. Ma Y, Hou Y, Zou L, et al. Composition analysis of different germplasm of Pseudostellaria heterophylla. Zhong Hua Zhong Yi Yao Za Zhi 2015; 30: 2149-52.
9. Song Y, Lin D, Mei Q, et al. Research progress on chemical constituents and pharmacological effects of Radix Pseudostellariae. Zhong Guo Yao Shi 2019; 22: 1506-10.
10. Ma Y, Liu X, Wang Y, et al. Research progress on main chemical constituents and pharmacological activities of Adenophora littoralis. Jilin Yi Yao Xue Yuan Xue Bao 2019; 40: 357-9.
11. Liu L, Jia J, Zeng G, et al. Studies on immunoregulatory and anti-tumor activities of a polysaccharide from Salvia miltiorrhiza Bunge. Carbohydr Polym 2013; 92: 479-83.
12. Ding F, Wang Y, Zhang Y. Research progress on pharmacological action and clinical application of active components of Salvia miltiorrhiza Bunge. Zhong Hua Zhong Yi Yao Za Zhi 2021; 36: 659-62.
13. Liu W, Jiang Q. Theoretical analysis of the pathogenesis of rheumatoid arthritis “dampness, heat and blood stasis”. Zhong Yi Za Zhi 2020; 61: 2148-53.
14. Liu X, Li X, Li X, et al. The efficacy and safety of total glucosides of peony in the treatment of primary Sj?gren’s syndrome: a multi-center, randomized, double-blinded, placebo-controlled clinical trial. Clin Rheumatol 2019; 38: 657-64.
15. Liu G, Wang Z, Li X, et al. Total glucosides of paeony (TGP) alleviates constipation and intestinal inflammation in mice induced by Sj?gren’s syndrome. J Ethnopharmacol 2020; 260: 113056.
16. Feng R, Chen J, Liu C, et al. A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice. Phytomedicine 2019; 58: 152824.
17. Wang S, Zhang S, Wang S, Gao P, Dai L. A comprehensive review on Pueraria: insights on its chemistry and medicinal value. Biomed Pharmacother 2020; 131: 110734.
18. Chen X, Yu J, Shi J. Management of diabetes mellitus with puerarin, a natural isoflavone from Pueraria lobata. Am J Chin Med 2019; 46: 1771-89.
19. Son Y, Kook S, Lee J. Glycoproteins and polysaccharides are the main class of active constituents required for lymphocyte stimulation and antigen-specific immune response induction by traditional medicinal herbal plants. J Med Food 2017; 20: 1011-21.
20. Gu SH, Kong WS, Zhang T, et al. Chemical constituents and pharmacological effects of Atractylodes macrocephala Koidz. Progress of compound clinical application. Zhong Hua Zhong Yi Yao Xue Kan 2020; 38: 69-73.
21. Du X, Bai Y, Shen H. Hu Yinqi’s experience in treating primary Sjogren’s syndrome from toxin, blood stasis and deficiency. Zhong Yi Za Zhi 2020; 61: 1594-7.
22. Xu X, Lü H, Li X, Su H, Zhang X, Yang J. Danshen attenuates osteoarthritis-related cartilage degeneration through inhibition of NF-κB signaling pathway in vivo and in vitro. Biochem Cell Biol 2017; 95: 644-51.
23. Ma L, Tian G, Geng H, et al. Brief introduction of TCM pharmacology database TCMSP and its analytical application. Zhong Guo Xun Zheng Xin Xue Guan Yi Xue Za Zhi 2020; 12: 1413-6.
24. Luo J. A brief introduction to UniProt. Sheng Wu Xin Xi Xue 2019; 17: 131-4.
25. Zhou Y, Su S. Research methods and progress of compatibility of Traditional Chinese Medicine. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2019; 25: 202-8.
26. Liu S, Wang W, Lu F, et al. Retro-origin and modern research review of the theory of medicinal properties of Traditional Chinese Medicine. Zhong Cao Yao 2020; 51: 5099-04.
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