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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 4: 661 - 666

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.04.003

Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

  • Bin ZHU ,
  • Ning YU ,
  • Lei WANG ,
  • Yue TIAN ,
  • Mingfen WU ,
  • Zhigang ZHAO
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  • 1 Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China
    2 Accreditation Center of Traditional Chinese Medicine Physician of National Administration of Traditional Chinese Medicine, Beijing 100120, China
    3 Department of Endocrinology, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100050, China

Received date: 2022-03-16

  Accepted date: 2022-07-08

  Online published: 2023-07-03

Supported by

Natural Science Foundation of China: the Protective Effect of Schizandrin A to β Cell Apoptosis by DNase I in Type 2 Diabetes(81803909)

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Abstract

OBJECTIVE: To explore the synergistic effect of deoxyribonuclease I (DNase I) knockdown combined with Schizandrin A (Sch A) in protecting islet beta-cells (β-cells) from apoptosis under high-glucose (HG) conditions.

METHODS: The concentration of Sch A was detected by Cell Counting Kit-8 (CCK-8). High glucose-cultured rat insulinoma beta cell line (RIN-M5F) cells were treated with Sch A and transfected with DNase I small interfering RNA (siRNA). Cell apoptosis rate and apoptosis-related protein level were examined by flow cytometry and Western blot method respectively. In addition, Na+-K+-adenosine triphosphatease (Na+-K+-ATPase) and Ca2+-Mg2+-ATPase activity, cell membrane potential, and intracellular Ca2+ concentration was also examined respectively.

RESULTS: Our study revealed that HG stimulation can cause a significant increase in DNase I level and cell apoptosis rate. However, Sch A combined with DNase I knockdown can significantly decrease the cell apoptosis rate and apoptosis-related protein levels such as BAX (P < 0.05) and Caspase-3 (P < 0.01). In addition, we also found that the combination of Sch A and DNase I knockdown can dramatically increase cell membrane potential level, Na+-K+-ATPase, and Ca2+-Mg2+-ATPase activity. Meanwhile, intracellular Ca2+ concentration was also found to be significantly decreased by the synergistic effect of Sch A and DNase I knockdown.

CONCLUSION: Overall, our study reveals a synergistic effect of Sch A and DNase I knockdown in protecting β-cells from HG-induced apoptosis.

Key words： high glucose; diabetes; β-cell apoptosis; synergistic effect; Schizandrin A

Cite this article

Bin ZHU , Ning YU , Lei WANG , Yue TIAN , Mingfen WU , Zhigang ZHAO . Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration[J]. Journal of Traditional Chinese Medicine, 2023 , 43(4) : 661 -666 . DOI: 10.19852/j.cnki.jtcm.2023.04.003

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