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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 3: 542 - 551

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.03.004

Mechanism underlying Fanmugua (Fructus Caricae) leaf multicomponent synergistic therapy for anemia: data mining based on hematopoietic network

  • Lihong JIA ,
  • Defu TIE ,
  • Zhaohui FAN ,
  • Dan CHEN ,
  • Qizhu CHEN ,
  • Jun CHEN ,
  • Huaben BO
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  • 1 School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, Guangdong 510006, China
    2 Department of Pharmacy, Zhanjiang Health School of Guangdong, Guangdong 524037, China
    3 College of Pharmacy, Guangdong Pharmaceutical University, Guangdong 510006, China

Received date: 2022-01-19

  Accepted date: 2022-04-18

  Online published: 2023-04-28

Supported by

Study on the Effect of Intestinal Bacteria on the Hematopoietic Component Absorption Mechanism of Danggui Buxue Tang(82104514);Based on the Multilevel Pharmacodynamic Model to Explore the Material Basis of Regulation of Erythroid Progenitor Cell Differentiation by Danggui Buxue Tang(201707010441);Innovation and University Promotion Project of Guangdong Pharmaceutical University(2017KCXTD020)

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Abstract

OBJECTIVE: To investigate the underlying mechanism of Fanmugua (Fructus Caricae) Leaf (CPL) multicomponent synergistic therapy for anemia.

METHODS: The components were identified in the literature. Six databases were searched for targets of CPL. Enrichment analysis was used to determine the targets associated with anemia and in bone marrow. Based on the Kyoto Encyclopedia of Genes and Genomes database, pathways and targets related to hematopoiesis were obtained. The key targets were obtained by protein-protein interaction analysis. Molecular docking was used to analyze the binding ability of key targets and active components. Bone marrow cells were used as an experimental model to verify the drug efficacy.

RESULTS: A total of 139 components and 1868 targets of CPL were retrieved from the literature. By disease enrichment analysis, 543 targets for hemorrhagic anemia, 223 targets for aplastic anemia, and 126 targets for sickle cell anemia were obtained. Target organ enrichment yielded 27, 29, and 20 targets of bone marrow. Based on KEGG pathway enrichment, a total of 47 shared hematopoietic pathways and 42 related targets were found. The key targets were vascular endothelial growth factor A (VEGFA), interleukin 10 (IL-10), platelet-endothelial cell adhesion molecule-1 (PECAM1), C-C motif chemokine 2 (CCL2), and vascular cell adhesion molecule 1 (VCAM1). The CPL active components included ursolic acid, quercetin, and hesperidin. The expression of VEGFA was significantly increased after CPL treatment. Quercetin and ursolic acid acted on VEGFA. Quercetin and Hesperidin acted on VCAM1. Quercetin acted on IL-10, CCL2, VCAM1, and VEGFA. Cell experiments revealed that CPL could promote the proliferation and migration of bone marrow cells.

CONCLUSIONS: CPL has the synergistic efficacy of treating anemia through multiple components, targets, and pathways.

Key words： anemia; data mining; network pharmacology; molecular docking simulation; Carica papaya (Fructus Caricae) Leaf; synergistic effect

Cite this article

Lihong JIA , Defu TIE , Zhaohui FAN , Dan CHEN , Qizhu CHEN , Jun CHEN , Huaben BO . Mechanism underlying Fanmugua (Fructus Caricae) leaf multicomponent synergistic therapy for anemia: data mining based on hematopoietic network[J]. Journal of Traditional Chinese Medicine, 2023 , 43(3) : 542 -551 . DOI: 10.19852/j.cnki.jtcm.2023.03.004

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