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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 3: 507 - 513

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230214.004

Efficacy of Danggui Buxue decoction (当归补血汤) on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism

  • Ye ZHAO ,
  • Xian WANG ,
  • Ling GU ,
  • Zihang LI ,
  • Jingtian ZHU ,
  • Wenkai WANG ,
  • Liang ZHANG ,
  • Mei XUE
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  • 1 School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China
    2 College of Traditional Chinese Medicine?College of Intergrated Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China

Received date: 2022-01-23

  Accepted date: 2022-05-25

  Online published: 2023-02-14

Supported by

Research on the Mechanism of Danggui Buxue Decoction in the Treatment of Diabetic Renal Interstitial Fibrosis Based on the Regulation of TGF-β1/Smad3 Signaling Pathway by MiRNA-27a(NZY81904085)

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Abstract

OBJECTIVE: To observe the efficacy of Danggui Buxue decoction (当归补血汤, DBD) on diabetic nephropathy-induced renal fibrosis in rats, and to study the possible mechanism.

METHODS: Sixty male Goto Kakizaki (GK) rats were randomly assigned to the model group, gliquidone group, astragaloside IV group, and high-, medium- and low-doses DBD groups. After 8 weeks, changes in body weight, blood glucose, serum creatinine, serum urea nitrogen, and total cholesterol were observed. Changes in transforming growth factor-β1 (TGF-β1), Smad3, and Smad5 pathways and the expression of the fibrosis-related proteins collagen IV (col IV), α-smooth muscle actin (α-SMA), and vimentin were assessed. The degree of renal fibrosis was observed by immunohistochemistry and Mason staining. The expression of interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor (TNF-α), and C-reactive protein (CRP) in the kidneys was assessed using enzyme linked immunosorbent assay.

RESULTS: Our experiments showed that DBD effectively reduced blood glucose, blood urea nitrogen, and creatinine levels after 8 weeks of administration, improved renal function in diabetic rats, alleviated renal fibrosis, and reduced the renal tissue levels of IL-6, IL-10, TNF-α, and CRP. Furthermore, DBD decreased the expression of TGF-β1, Smad3, col IV, α-SMA, and vimentin in renal tissues and increased the expression of Smad5.

CONCLUSIONS: DBD ameliorates diabetic renal interstitial fibrosis by modulating the TGF-β1/Smads pathway.

Key words： Danggui Buxue decoction; interleukins; transforming growth factor beta1; Smad proteins; C-reactive protein; renal fibrosis

Cite this article

Ye ZHAO , Xian WANG , Ling GU , Zihang LI , Jingtian ZHU , Wenkai WANG , Liang ZHANG , Mei XUE . Efficacy of Danggui Buxue decoction (当归补血汤) on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism[J]. Journal of Traditional Chinese Medicine, 2023 , 43(3) : 507 -513 . DOI: 10.19852/j.cnki.jtcm.20230214.004

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