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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 3: 466 - 473

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230214.001

Sanwu Baisan decoction (三物白散) inhibits colorectal cancer progression in mice by remodeling gut microbiota and tumorigenesis

  • Yiqian JIANG ,
  • Xibin ZHOU ,
  • Wenyuan PU ,
  • Chunxiang ZHOU
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  • 1 Department of Febrile Disease, Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, China
    2 Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China

Received date: 2022-02-18

  Accepted date: 2022-05-25

  Online published: 2023-02-14

Supported by

National Natural Science Foundation-funded Project: the Biological Mechanism of Lingguizhugan Decoction Treating Alzheimer's Disease via Regulating Brain Lymphatic Drainage System(82074504);Priority Academic Program Development of Jiangsu Higher Education Institutions (Integration of Chinese and Western Medicine);Natural Science Foundation of the Jiangsu Higher Education Institutions-funded Project: to Explore the Mechanism of Sanwubai San Regulating Gut Microbiota Against Colorectal Cancer By IL-23/ΓδT17/IL-17 Axis(19KJB360003)

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Abstract

OBJECTIVE: To uncover the anti-tumor effects and potential mechanism of Sanwu Baisan Decoction (三物白散, SWB) in treatment of colorectal cancer (CRC) in mice.

METHODS: Therapeutic effect was evaluated based on body weight gain, tumor volume, tumor growth inhibition rate, and histological changes and apoptosis in the tumor tissues. Anti-tumor immunity was studied by measuring plasma levels of anti-tumor cytokines, interleukin 6 (IL-6), interleukin 17 (IL-17), and interferon γ (IFN-γ). Gut morphological changes were evaluated by histological staining and tight junction proteins expressions. Gut microbiota composition was analyzed by 16S rRNA gene sequencing. Classical toll-like receptor 4 (TLR-4)/ cyclooxygenase 2 (COX-2)/ prostaglandin E2 (PGE-2) pathway was examined in colon tissue and tumor samples.

RESULTS: SWB presented high anti-tumor efficacy of CRC in mice, which manifested as decreased tumor volume and increased tumor growth inhibition rate. This anti-tumor effect of SWB was associated with elevated plasma levels of anti-tumor immune cytokines (IL-6, IL-17, and IFN-γ). Further studies showed that SWB also increases the expression of occluding and promotes the abundance of gut probiotics, Clostridium_XIVa, Enterorhabdus, and uncultured_bacterium. Moreover, results suggested that the anti-tumor effects of SWB might associate with inducing cancer cell apoptosis and inhibiting the TLR-4/COX-2/PGE-2 pathway in both colon tissue and tumor samples.

CONCLUSION: SWB shows strong anti-tumor efficiency in mice with colorectal carcinoma, possibly through promoting the secretion of anti-tumor immune cytokines, inducing cancer apoptosis, maintaining the gut microbiota, and inhibiting tumorigenesis by inhibiting the TLR-4/COX-2/PGE-2 pathway.

Key words： colorectal neoplasms; carcinogenesis; apoptosis; gastrointestinal microbiome; dysbiosis; Sanwu Baisan decoction

Cite this article

Yiqian JIANG , Xibin ZHOU , Wenyuan PU , Chunxiang ZHOU . Sanwu Baisan decoction (三物白散) inhibits colorectal cancer progression in mice by remodeling gut microbiota and tumorigenesis[J]. Journal of Traditional Chinese Medicine, 2023 , 43(3) : 466 -473 . DOI: 10.19852/j.cnki.jtcm.20230214.001

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