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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 2: 416 - 428

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221214.001

Review

Wuzi Yanzong prescription (五子衍宗丸) from Traditional Chinese Medicine for male infertility: a narrative review

  • Zhimin CHEN ,
  • Mingyue AO ,
  • Yujiao LIAO ,
  • Lingying YU ,
  • Zhuo YANG ,
  • Lin HU ,
  • Wenbing LI ,
  • Changjiang HU ,
  • Yongxiang GAO
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  • 1 Department of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
    2 Department of Pharmacy, Chengdu Women’s & Children’s Central Hospital, Chengdu 610074, China
    3 Institute of Qinghai-tibetan Plateau, Southwest Minzu University, Chengdu 610041, China

Received date: 2021-11-19

  Accepted date: 2022-03-17

  Online published: 2023-03-14

Supported by

National Natural Science Foundation of China: Study on the Mechanism of “Stir-frying with Salt-water Into the Kidney, Kidney Governing Reproduction” Based on Composition of Wuzi Yanzong Wan Before and After Salted(81773899);Fellowship of China Postdoctoral Science Foundation: Study on the Material Basis and Mechanism of Huangsi Yujin Vinegar for “Soothing the Liver and Relieving Pain”(2020M673567XB);Key R & D projects of Science & Technology Department of Sichuan Province: National Intangible Cultural Heritage-Protection, Inheritance and Development of Sichuan Traditional Chinese Medicine Processing Skills(2020YFS0359);Xinglin Scholar Research Premotion Project of Chengdu University of TCM: Research on the Scientific Connotation of Wuzi Yanzong Pill Processing Efficiency Enhancement Based on “Composition Target Path”(BSH2019026);Open Research Fund of Chengdu University of Traditional Chinese Medicine Key Laboratory of Systematic Research of Distinctive Chinese Medicine Resources in Southwest China: Study on the Scientific Connotation of Wuzi Yanzong Pill Processing(2020BSH012)

Fold

Abstract

Wuzi Yanzong prescription (WYP), as a classical prescription for male infertility with kidney essence deficiency, is composed of Gouqizi (Fructus Lycii), Tusizi (Semen Cuscutae), Wuweizi (Fructus Schisandrae Chinensis), Fupenzi (Fructus Rubi Chingii) and Cheqianzi (Semen Plantaginis). It has been used for hundreds of years in the treatment of male infertility, known as “the first prescription of ancient and modern seeds”, with convincing clinical evidence. At present, more than 100 chemical compounds have been isolated from WYP, including polysaccharide, fatty acids, flavonoids, phenylpropanoids, organic acids, alkaloids, terpenoids, etc. Pharmacological and clinical studies show that WYP has an obvious effect on reproductive system diseases, especially male infertility, which has a very wide application prospect. It also has effects on the nervous system, inhibiting liver injury, lowering blood sugar and blood lipid, anti-aging, improving immunity, resisting hypoxia and fatigue effects. This study reviewed the chemical constituents, quality control, pharmacology, and clinical application of WYP. There is no doubt about the clinical value of WYP, but its quality control system is not perfect, pharmacological mechanism is not fully explained, and clinical applications need to be reevaluated. Therefore, the follow-up researches should proceed from the theory of Traditional Chinese Medicine (TCM) and clinical applications, further explain the theoretical connotation, reveal the mechanism of action, and provide the basis for the secondary development of classic famous prescriptions. In addition, WYP is mostly used in combination with western medicines besides being used alone. Whether it can improve the efficacy and reduce side effects will also be a meaningful research direction in the future.

Key words： quality control; pharmacology; clinical applications; chemical constituents; Wuzi Yanzong prescription; review

Cite this article

Zhimin CHEN , Mingyue AO , Yujiao LIAO , Lingying YU , Zhuo YANG , Lin HU , Wenbing LI , Changjiang HU , Yongxiang GAO . Wuzi Yanzong prescription (五子衍宗丸) from Traditional Chinese Medicine for male infertility: a narrative review[J]. Journal of Traditional Chinese Medicine, 2023 , 43(2) : 416 -428 . DOI: 10.19852/j.cnki.jtcm.20221214.001

References

1. Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med 2012; 9: e1001356.
2. Tang W, Hu DX. Application and progress of stem cells in male infertility. Di San Jun Yi Da Xue Xue Bao 2019; 41:1897-901.
3. Li L, Wang TS. Historical changes and modern pharmacological research of Wuziyanzong pill. Zhong Yi Yao Lin Chuang Za Zhi 2018; 30: 608-11.
4. Han DC. Representative prescription for tonifying kidney-Wuziyanzong pill. Zhong Hua Zhong Yi Yao Za Zhi 2017; 22: 26.
5. Xu HJ, Liu Q, Guo YL, Gao F, Li M, Wei PF. Research progress of Wuzi Yanzong pill in the treatment of male infertility oligoasthenospermia. Liaoning Zhong Yi Yao Da Xue Xue Bao 2020; 22: 149-53.
6. Wang YY, Wang L, Qi YX, Liu Q, Zhang YQ. Research progress on chemical constituents of Cuscutae Semen. Shandong Zhong Yi Yao Da Xue Xue Bao 2020; 44: 705-12.
7. Zhang F, Guo S, Qian DW, Zhang X, Zhang WH, Duan JA. Research and development status and prospect analysis of multiple types of small molecule chemicals in Lycium barbarum. Zhong Cao Yao 2016; 39: 2917-21.
8. Bai WY, Wang HE, Wang BY, Yu HS, Li Z. Research progress on chemical constituents and pharmacological effects of Schisandra chinensis. Zhong Cheng Yao 2019; 41: 2177-83.
9. Cheng D, Li J, Zhou B, Zhen PW. Research progress on chemical constituents and pharmacological effects of Rubi Fructus. Zhong Yao Cai 2012; 35: 1873-76.
10. Li CC, Gong SX, Xu J, et al. Research progress on chemical constituents and pharmacological effects of Semen Plantaginis and prediction analysis of quality markers. Zhong Cao Yao 2018; 49: 1233-46.
11. Xu S, Xu WF, Liang XL, Wu XJ, Jin PF. Research progress on chemical constituents and biological activities of Semen Plantaginis. Xi Bei Yao Xue Za Zhi 2019; 34: 567-70.
12. Fu WH, Yu M. Research progress of Gouqizi (Fructus Lycii). Shi Jie Zui Xin Yi Xue Xin Xi Wen Zhai 2017; 17: 104.
13. Li SS, Qi YL, Hua M, Sun YS. Separation, purification and antioxidant activity of polysaccharides from Schisandra chinensis. Shi Pin Gong Ye 2018; 39: 233-7.
14. Cui L, Zheng ZQ. Research progress on chemical constituents and pharmacological effects of Rubi Fructus. Quan Ke Kou Qiang Yi Xue Dian Zi Za Zhi 2020; 7: 192+6.
15. Zhang Q, Chen W, Zhao J, Xi W. Functional constituents and antioxidant activities of eight Chinese native goji genotypes. Food Chem 2016; 200: 230-6..
16. Yang Q, Qu XB, Li H, et al. Research progress on chemical constituents and pharmacological effects of Schisandra chinensis. Jilin Zhong Yi Yao 2015; 35: 626-8.
17. Zhang HH, Huang JP, Li W, et al. Evaluation of Wuzi Yanzong decoction in the treatment of mild cognitive dysfunction in Parkinson's disease with kidney deficiency and marrow reduction. Zhejiang Zhong Xi Yi Jie He Za Zhi 2016; 26: 998-1001.
18. Xie M, Yang SS, Wang LL, Li L, Wang XY. Research progress of Plantaginis Semen. Heilongjiang Yi Yao 2015; 28: 474-6.
19. Xiang SX, He ZS, Ye Y. Four furan lignans from Cuscuta chinensis. Chin J Chem 2010; 19: 282-5.
20. Tan W, Huang XH, Fu TT, Qiu WZ, Min CY, Wang CJ. Analysis of fatty acids in Wuzi Yanzong pills by GC-MS. Xian Dai Yi Yuan 2016; 16: 1186-8 + 91.
21. Zou DX, Wang JF, Zhang B, et al. Analysis of chemical constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS. Molecules 2015; 20: 21373-404.
22. Yan JN, Hu C, Meng XL. Pharmacodynamic mechanism of Wuzi Yanzong pill based on network pharmacology. Shi Jie Ke Xue Ji Shu 2020; 22: 2294-310.
23. Zhou JL, Liu W, Tan CM, Zhu Ming, Ma SC. Study on quality control standard of Wuzi Yanzong pills. Zhong Guo Yao Xue Za Zhi 2015; 50: 125-130.
24. He CX, Yuan D, He YM, et al. Application of multiple evaluation method in quality control of Wuzi Yanzong pills. Zhong Cao Yao 2017; 48: 3754-9.
25. Cai JA. Determination of betaine in Wuzi Yanzong pills by HPLC. Zhong Guo Zhong Yi Yao Xin Xi Za Zhi 2011; 18: 57-8.
26. Zhou JL, Liu W, Chen BL, Zhu M. Determination of scopoletin in Gouqizi and Wuziyanzong pills by HPLC with fluorescence detection. Zhong Guo Xian Dai Ying Yong Yao Xue 2015; 32: 482-6.
27. Dong QJ, Zhou Y, Feng W, et al. Application of fingerprint combined with multiple evaluation method in quality evaluation of Wuzi Yanzong pills. Zhong Yao Cai 2019; 42: 579-83.
28. Dong QJ, Wang XG, Niu LY, Feng W. Study on fingerprints of Wuzi Yanzong prescription-medicinal materials based on peak pattern matching. Zhong Cao Yao 2017; 48: 1153-8.
29. Liu W, Zhou JL, Chen BL, Zhu M. Study on HPLC fingerprint of Wuzi yanzong Pills. Yi Xue Dao Bao 2014; 33: 1360-4.
30. Liu MM, Zhang CC, Jia LL, et al. Protective effect of Wuzi Yanzong recipe on oxidative damage of testis in mice induced by cyclophosphamide. Zhong Cheng Yao 2013; 35: 2591-7.
31. Meng XD, Zhao CJ, Zhao X, et al. Effect of Wuzi Yanzong Wan on testicular gene expression profile of mice with oligospermia. Huan Qiu Zhong Yi Yao 2020; 13: 1653-60.
32. Zou Y, Zhao FF, Li H, et al. Intervention of Wuzi Yanzong pills on the reproductive function of AT1KO male mice. Shanghai Zhong Yi Yao Za Zhi 2020; 54: 97-100.
33. Liao YJ, Zhang X, Ao MY, Hu CJ, Chen ZM, Xu RC. Effect of prescription on spermatogenic cell apoptosis in rats after preparation of drug salt contained in Wuziyanzong pill. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2021; 27: 1-7.
34. Yan RH, Huo JF, Dong AG, Wang YH, Liang Q. Comparative study on the effect of reproductive nutrition capsule and Wuzi Yanzong pill on reproductive function of drug-induced oligospermia model. Zhong Hua Zhong Yi Yao Xue Kan 2015; 33: 1215-7+99.
35. Cui TW, Liu BX, Qin M, Zhang XP, Gao YX. The effect of Wuzi Yanzong pills containing serum on the secretory function and autophagy of mouse Sertoli cells. Zhong Hua Zhong Yi Yao Za Zhi 2107; 32: 549-52.
36. Yin JL, Xu Y, Wu B. The effect of Wuzi Yanzong compound on oxidative stress injury and apoptosis of testicular Sertoli cells. Zhong Hua Nan Ke Xue Za Zhi 2013; 19: 257-61.
37. Liu SJ, Chen YC, Hu SQ, Li CR, Guo J. Protective effect and mechanism of Wuzi Yanzong pill on testis of heat stressed rats. Beijing Zhong Yi Yao Da Xue Xue Bao 2020; 43: 386-92.
38. Li L, Dai Ni, Na S, et al. Protective effect and mechanism of Wuzi Yanzong pills on experimental oligoasthenospermia rats. Zhong Hua Nan Ke Xue Za Zhi 2016; 22: 827- 33.
39. Liu HJ, Wu DL, Tong XH, Li L, Wang TS. Mechanism of Wuzi Yanzong pills interfering with mitochondrial permeability transition pore to inhibit sperm apoptosis. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2020; 26: 34-9.
40. Chen Y, Fu ZY, Zhang YG, Peng JL. Wuzi Yanzong Wan regulates the S100A11 gene during implantation in GnRHa controlled hyperovulation mice. Zhong Yi Yao Dao Bao 2014; 20: 14-7.
41. Wang YX, Ma N, Zhong XM, Cui R, Miao ZL. Study on the effect of total flavonoids of Cuscuta on ovarian function in rats with premature ovarian failure. Yi Xue Zong Shu 2019; 25: 2695-9.
42. Miao MS, Peng MF, Yan XL, et al. The effect of total flavonoids of Cuscuta on the rat model of polycystic ovary syndrome induced by letrozole. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2109; 25: 17-23.
43. Zeng KW, Zhang T, Fu H, Liu GX, Wang XM. Modified Wu-Zi-Yan-Zong prescription, a traditional Chinese polyherbal formula, suppresses lipopolysaccharide-induced neuroinflammatory processes in rat astrocytes via NF-κB and JNK/p38 MAPK signaling pathways. Phytomedicine 2012; 19: 122-9.
44. Zhang RN, Chai Z, Fan HJ, et al. Study on the prevention and treatment effects of Wuzi Yanzong pills on mice with experimental autoimmune encephalomyelitis and its mechanism. Zhong Hua Zhong Yi Yao Za Zhi 2018; 33: 1316-9.
45. Sun MY, Fan HJ, Chai Z, et al. Effect of Wuziyanzong Wan on neurotrophic factor secreted by astrocytes in demyelinating mice. Zhong Yi Za Zhi 2021; 62:712-6.
46. Liu X, Sun MY, Xue JY, Li YR, Fan HJ, Chai Z. Inhibitory effect of Wuziyanzong pill on inflammatory response in demyelinating model. Zhong Hua Zhong Yi Yao Za Zhi 2021; 36: 2904-8.
47. Li YL, Fan HJ, Chai Z, et al. Neuroprotective and anti-inflammatory effects of Wuzi Yanzong pills on Parkinson's disease mice. Zhong Hua Zhong Yi Yao Za Zhi 2020; 35: 3623-6.
48. Li RX, Fan HJ, Cai Z, et al. Effects of Wuzi Yanzong pills (五子衍宗丸) on the apoptotic pathway of neural tube defects cell model. Zhong Yi Za Zhi 2019; 60: 1134-41.
49. Song FJ, Zeng KW, Yu Q, Song YX, Tu PF, Wang XM. Study of modified Wuzi-Yanzong prescription's effect on gene expression profile of SAMP8 mice brain based on gene microarray. Zhong Guo Yao Xue Za Zhi 2015; 50: 1874-9.
50. Yang CJ, Fan HJ, Chai Z, et al. Mechanism of Wuzi Yanzong pill in preventing neural tube malformation. Zhong Guo Zhong Yi Ji Chu Yi Xue Za Zhi 2021; 27: 1887-91.
51. Chen WC, Tan LH, Li L. Analysis of patent information of Traditional Chinese Medicine for the treatment of urinary system diseases in China. Zhong Guo Fa Ming Yu Zhuan Li 2021; 18: 44-9.
52. Bae S, Lee KW, Jeong HC, et al. Effects of a combination of herbal extracts [modified Ojayeonjonghwan (Wuzi Yanzong wan)] on partial urethral obstruction-induced detrusor overactivity in rats: impact on the nitric oxide pathway and oxidative stress. BMC Complement Altern Med 2019; 19: 64.
53. Ge ZY, Jin L, He FJ, Dong XX, Li HK, Liu JX. Experimental study of Wuziyanzong pill on oligospermia model rats. Sheng Zhi Yi Xue Za Zhi 2010; 19: 224-7.
54. Zhang X, Liao YJ, Yang Z, Chen ZM, Li WB, Hu CJ. Study on the changes of chemical components and pharmacodynamics of Rubi Fructus before and after salt preparation. Shi Zhen Guo Yi Guo Yao 2020; 31: 82-4.
55. Wu QM, Ni HX, Lu X, Yuan X, Luo YY, Jin YM. Kaempferol delays kidney injury in mice with spontaneous obesity type 2 dia-betes mellitus. Xin Nao Xin Guan Bing Fang Zhi 2017; 17: 19-22.
56. Tang LH, Fang C, Wang HR, Tang SG. Protective effects of kaempferol on renal function and histopathological damage in rats with diabetic nephropathy induced by high glucose. Mian Yi Xue Za Zhi 2018; 34: 1041-6.
57. Qiu SJ. The protective effect and mechanism of ISO rhamnin on diabetic rats. Jinan: Shandong University, 2017: 35-46.
58. Han GF, Zhang CC, Chen X, Chen YQ, Yuan D, Zhao HX. Extraction of total polysaccharides from Wuzi Yanzong prescription and its protective effect on liver injury. Shi Zhen Guo Yi Guo Yao 2017; 28: 549-51.
59. Liu ZC, Zhao HX, Chen X, Liu GY, Yuan D, Zhang CC. Protective effect and mechanism of Wuzi-Yanzong prescription against the liver injury induced by cyclophosphamide. Zhong Guo Quan Ke Yi Xue 2017; 20: 3426-30.
60. Yang Z, Zhang X, Hu CJ. The effect of Wuzi Yanzong pills on the immune function of rats with kidney essence deficiency after being prepared with salt. Shi Zhen Guo Yi Guo Yao 2019; 30: 2134-7.
61. Zhu XD, Wang Y. Effect of Wuzi Yanzong pill on lens oxidative damage and polyol pathway in diabetic cataract mice. Zhong Yi Yan Jiu 2013; 26: 66-9.
62. Yuan Y, Wang XH. The effect of Wuzi Yanzong pills on anti-fatigue and hypoxia tolerance in mice. Yao Xue Shi Jian Za Zhi 2008; 26: 430-1.
63. Fu TT, Li J, Wang CJ, Tan W. Study on the mechanism of Wuzi Yanzong pill interfering with SIRT1 signal pathway to regulate tumor related fatigue. Zhong Yao Cai 2017; 40: 2657-62.
64. Li JJ, Dong L, Tan K, Yu Yan, Yu XJ, Yang F. Clinical study on improving sperm DNA integrity damage by tonifying the kidney and activating blood circulation. Ya Tai Chuan Tong Yi Yao 2021; 17: 79-83.
65. Li H, Yu XJ, Yang F, Dong L, Tan K, Li JJ. Clinical study on 64 cases of male infertility treated with Wuzi Yanzong pill. Zhong Guo Xing Ke Xue 2020; 29: 123-6.
66. Yu WH, Zhan JF, Chen R. Clinical study of Jinshuibao capsule and Wuziyanzong pill in the treatment of male idiopathic oligoasthenospermia. Xin Zhong Yi 2021; 53: 88-92.
67. Yang D, Xian H, Teng WD, Cheng QJ. Clinical efficacy and safety of Wuzi Yanzong pill and Qilin pill in the treatment of male idiopathic oligoasthenospermia. Zhong Guo Xing Ke Xue 2019; 28: 77-80.
68. Li YC, Pan ES, Chen CP. Effect of Shengjing Zhongyu decoction on oligospermia and asthenospermia infertility and its influence on caspase 3 and caspase 8 in semen. Shi Jie Zhong Yi Yao 2019; 14: 2359-62+6.
69. Tan WJ, Han WW, Yan L. Clinical observation of Shugan Yiyang capsule in the treatment of abnormal semen liquefaction. Zhong Cheng Yao 2018; 40: 1227-30.
70. Zhang LJ, Luan HT. Modified Wuzi Yanzong pills in the treatment of 86 cases of adolescent dysfunctional uterine bleeding. Hebei Zhong Yi 2012; 34: 56-7.
71. Sun QF. Clinical study on Wuzi Yanzong pills in the treatment of ovulation dysfunction infertility. Jilin Zhong Yi Yao 2012; 32: 1243-4.
72. Wang K, Wang J, Liu XJ, Ma AW. The effect of Wuzi Yanzong pills on the endocrine and metabolism of infertility patients caused by luteal insufficiency due to kidney deficiency and essence deficiency. Zhong Yao Cai 2021; 44: 720-3.
73. Zhang LM, Liao BN, Zhou HG, Niu CQ. Jiawei Wuzi Yanzong pills in the treatment of female infertility caused by luteal insufficiency due to kidney deficiency and essence deficiency. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2017; 23: 197-202.
74. Li LR, Zhang XF, Pan SJ. Observation on the efficacy of Wuzi Yanzong pills combined with western medicine in the treatment of anovulatory infertility. Zhong Yi Lin Chuang Yan Jiu 2014; 6: 4-5.
75. HHe JQ, Liu FP, Huang YL, Deng LJ, Huang LH. Pregnancy promoting effect of Wuziyanzong pill combined with Western Medicine on patients with ovulatory infertility. Shi Jie Zhong Yi Yao 2021; 16: 1463-7.
76. Li WW, Wang LL, Fu H, Liu GX, Wang XM. Research status of neuroprotective mechanism of Jiawei Wuzi Yanzong formula. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2105; 21: 219-23.
77. Pan M, Li YJ, Wang HY. Wuzi Yanzong pills for the treatment of female urinary incontinence. Zhong Guo Lin Chuang Yan Jiu 2011; 24: 632-3.
78. Li L, Sun YX, Ma JY, Ning SS, Li Y. Clinical observation of compound Wuzi oral liquid in the treatment of female urethral syndrome. Shanghai Zhong Yi Yao Za Zhi 2018; 52: 62-4+ 8.
79. Wang XD. Clinical observation of Huangkui capsule combined with Wuziyanzong pill in the treatment of IgA nephropathy proteinuria damp heat syndrome. Shi Yong Zhong Yi Yao Za Zhi 2017; 33: 618-9.
80. Li G. Clinical observation on Wuzi Yanzong pill combined with moxibustion in the treatment of enuresis in children with deficiency cold syndrome of lower Yuan Dynasty. Shenzhen Zhong Xi Yi Jie He Za Zhi 2020; 30: 38-40.
81. Guo L. Clinical observation of Wuzi Yanzong pill combined with Western Medicine in the treatment of lung kidney Qi (Yang) deficiency in stable stage of chronic obstructive pulmonary disease. Beijing Zhong Yi Yao 2016; 35: 78-80.
82. Cao S, Li YP, Wu P, Tong L. Clinical research progress of Wuzi Yanzong pill. Ya Tai Chuan Tong Yi Yao 2017; 13: 55-6.
83. Ji LN, Zhang XY, Liang ZY, Shi BZ. Study on the effect of Jiawei Wuzi Yanzong pills on the outcome of assisted pregnancy in patients with low ovarian response. Zhong Guo Shi Yan Fang Ji Xue Za Zhi 2021; 27: 106-10.
84. Liu GN. Clinical efficacy analysis of Wuziyanzong pill in the treatment of liver injury after chemotherapy. Haerbin: Heilongjiang University of Traditional Chinese Medicine, 2018: 16-23.
85. Wang XL, Fan HJ, Chai Z, et al. Research progress in the mechanism of common monomers in Wuzi Yanzong pills on central nervous system diseases. Zhong Hua Zhong Yi Yao Xue Kan 2021; 39, 166-70.
86. Li GL. The mitigation effect of quercetin on the damage of diethylstilbestrol-induced spermatogenesis in hamsters. Baoding: Hebei Agricultural University, 2010: 15-22.
87. Zhang YW, Shao DY, Shi JL, Zhu J, Huang QS, Yang H. Research progress in the biological function of kaempferol. Sheng Ming Ke Xue 2017; 29: 400-5.
88. Wang HJ, Guo HJ, Zhang XY. The effect of kaempferol on HepG2 cell apoptosis. Wei Chang Bing Xue He Gan Bing Xue Za Zhi 2018; 27: 617-22.
89. Zhang YH, Li CM. Estrogen-like functions of phytosterols and their effects on animal reproduction and development. Xu Mu Yu Shou Yi 2011; 43: 103-7.
90. Hao HX, Xie XM, He JB. The effect of plant sterols on the growth and reproductive hormones of KM female mice. Xian Dai Xu Mu Shou Yi 2014; 305: 19-22.
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