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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 2: 312 - 321

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230124.001

Original articles

Danzhi Jiangtang capsule (丹蛭降糖胶囊) reduces renal injury in rats with diabetes induced by high fat diet and streptozotocin via downregulating toll-like receptor 4-nuclear factor-κB pathway and apoptosis

  • Jing XIE ,
  • Zheng BI ,
  • Sihai WANG ,
  • Guoming SHEN ,
  • Zhaohui FANG
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  • 1 Department of Endocrinology, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China
    2 Anhui Academic of Traditional Chinese Medicine Diabetes Research Institute, Hefei 230031, China

Received date: 2021-12-12

  Accepted date: 2022-03-08

  Online published: 2023-03-14

Supported by

Research Grant from the National Natural Science Foundation of China: Mechanism of Danzhi Jiangtang Capsule Regulating Adipocyte-derived Exosomes in the Prevention and Treatment of Type 2 Diabetes Mellitus Macrovascular Disease(82174153);Based on Chip Technology to Carry out Basic and Clinical Comparison to Explore the Research of Danzhi Jiangtang Capsule in Precise Treatment of Diabetic Macrovascular Disease(81774286)

Fold

Abstract

OBJECTIVE: To investigate the effect and mechanisms of Danzhi Jiangtang capsule (丹蛭降糖胶囊, DJC) on renal injury in streptozotocin (STZ)-induced diabetes of rats.

METHODS: Sprague-Dawley rats were fed with high fat diet for 6 weeks followed by streptozotocin (STZ, 35 mg/kg) injection. These rats were then treated with DJC (270, 540 and 1080 mg/kg) daily for 8 weeks.

RESULTS: A combination of high fat diet and STZ significantly increased blood glucose creatinine, urea nitrogen, and urine albumin in rats. Meanwhile, the glomerular and tubular lesions were observed in rats fed with high fat diet and injected with STZ. These biochemical and pathological changes were significantly attenuated by DJC treatments in a dose-dependent manner. Mechanistically, DJC treatments significantly decreased toll-like receptor 4 (TLR4), mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) signals in the kidney of rats fed with high fat diet and injected with STZ. Terminal deoxynucleotidyl transferase dUTP nick end labeling staining and caspase-8 levels showed that renal apoptosis was increased in rats fed with high fat diet and injected with STZ, and this was attenuated by DJC treatments.

CONCLUSIONS: DJC treatments protect against diabetic kidney disease, and the mechanism may be closely related to downregulation of TLR4/MAPK/NF-κB pathways and apoptosis. This study provides further evidence of using DJC as a potential therapeutic option for diabetic kidney disease.

Key words： diabetic nephropathies; inflammation; apoptosis; toll-like receptor 4; mitogen-activated protein kinase kinases; NF-kappa B; Danzhi Jiangtang capsule

Cite this article

Jing XIE , Zheng BI , Sihai WANG , Guoming SHEN , Zhaohui FANG . Danzhi Jiangtang capsule (丹蛭降糖胶囊) reduces renal injury in rats with diabetes induced by high fat diet and streptozotocin via downregulating toll-like receptor 4-nuclear factor-κB pathway and apoptosis[J]. Journal of Traditional Chinese Medicine, 2023 , 43(2) : 312 -321 . DOI: 10.19852/j.cnki.jtcm.20230124.001

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