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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 2: 252 - 264

DOI: https://doi.org/10.19852/j.cnki.jtcm.20230105.003

Original articles

In vitro assessment of antioxidant, neuroprotective, anti-urease and anti-tyrosinase capacities of Tamarix africana leaves extracts

  • Anissa Trad Khodja Esma ,
  • El Hamid Khabtane Abd ,
  • Arhab Rabah ,
  • Benouchenne Djamila ,
  • Sabri Bensaad Mohamed ,
  • Bensouici Chawki ,
  • Erenler Ramazan
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  • 1 Biotechnology, Water, Environment and Health Laboratory, Faculty of Natural and Life Sciences, University Abbes Laghrour, Khenchela 40000, Algeria
    2 Laboratory of Natural Substances, Biomolecules and Biotechnological Applications, Faculty of Natural and Life Sciences, University Larbi Ben M Hidi, Oum El-Bouaghi 04000, Algeria
    3 Biotechnology, Water, Environment and Health Laboratory. Faculty of Natural and Life Sciences, University Abbes Laghrour, Khenchela 40000, Algeria
    4 Laboratory of Natural Substances, Biomolecules and Biotechnological Applications, Faculty of Natural and Life Sciences, University Larbi Ben M Hidi, Oum El-Bouaghi 04000, Algeria
    5 Genetics, Biochemistry and Plant Biotechnology Laboratory, Faculty of Natural and Life Sciences, University Constantine 1, Constantine 25000, Algeria
    6 Pharmaceutical Sciences Research Center, Constantine 25000, Algeria
    7 Laboratory of Cellular and Molecular Physio-Toxicology-Pathology and Biomolecules (LPTPCMB), Faculty of Natural and Life Sciences, University Batna 2, Batna 05000, Algeria
    8 Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Faculty of Natural and Life Sciences University Batna 2, Batna 05000, Algeria
    9 Biotechnology Research Center Ali Mendjli UV 03, Constantine 25000, Algeria
    10 Department of Chemistry, Faculty of Science and Literature, University TokatGaziosmanpasa, Tokat 60250, Turkey

Received date: 2022-03-22

  Accepted date: 2022-06-18

  Online published: 2023-03-14

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Abstract

OBJECTIVE: To characterize the chemical profile of methanolic crude extract and its fractions (Ethyl acetate, n-butanol and aqueous) using liquid chromatography-mass spectrometry (LC-MS) analysis, to evaluate their biological and pharmacological properties: antioxidant (1, 1-diphenyl-2-pycrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic) (ABTS·+), galvinoxyle free radical scavenging, reducing power, phenanthroline and β carotene-linoleic acid bleaching assays), enzymes inhibitory ability against several enzymes [acetyl-cholinesterase (AChE), buthyrylcholinesterase (BChE), urease and tyrosinase].

METHODS: Secondary metabolites were extracted from Tamarix africana air-dried powdered leaves by maceration, the crude extract was fractionated using different solvents with different polarities (Ethyl acetate, n-butanol and aqueous). The amount of polyphenols, flavonoids and tannins (hydrolysable and condensed) were determined using colorimetric assays. A variety of in vitro biochemical tests were carried out to assess antioxidant and oxygen radical scavenging properties using DPPH, ABTS, galvinoxyle free radical scavenging, reducing power, phenanthroline and β carotene-linoleic acid bleaching methods. Neuroprotective effect was examined against acetylcholinesterase and buthy-rylcholinesterase enzymes. The anti-urease and anti-tyrosinase activities were performed against urease and tyrosinase enzymes respectively. The extract's components were identified using LC-MS and compared to reference substances.

RESULTS: The results indicated that Tamarix africana extracts presented a powerful antioxidant activity in all assays and exhibited a potent inhibitory effect against AChE and BChE as well as urease and tyrosinase enzymes. LC-MS analysis identified amount of eight phenolic compounds were revealed in this analysis; Apigenin, Diosmin, Quercetin, Quercetine-3-glycoside, Apigenin 7-O glycoside, Rutin, Neohesperidin and Wogonin in methanolic extract and its different fractions of Tamarix africana from leaves.

CONCLUSIONS: Based on these findings, it is reasonable to assume that Tamarix africana could be considered as a potential candidate for pharmaceutical, cosmetics, and food industries to create innovative health-promoting drugs.

Key words： antioxidants; anti-tyrosinase; anti-urease; liquid chromatography-mass spectrometry; neuroprotection; phenolic content; Tamarix africana

Cite this article

Anissa Trad Khodja Esma , El Hamid Khabtane Abd , Arhab Rabah , Benouchenne Djamila , Sabri Bensaad Mohamed , Bensouici Chawki , Erenler Ramazan . In vitro assessment of antioxidant, neuroprotective, anti-urease and anti-tyrosinase capacities of Tamarix africana leaves extracts[J]. Journal of Traditional Chinese Medicine, 2023 , 43(2) : 252 -264 . DOI: 10.19852/j.cnki.jtcm.20230105.003

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