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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 1: 78 - 86

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220727.001

Original articles

Chinese herbal compound Jinsiwei (金思维) improves synaptic plasticity in mice with sporadic Alzheimer’s disease induced by streptozotocin

  • Meijing TIAN ,
  • Yannan HE ,
  • Mingcui ZHENG ,
  • Gaofeng QIN ,
  • Zhuoyan GONG ,
  • Shuaiyang HUANG ,
  • Pengwen WANG
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  • 1 Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
    2 Department of Respiratory Medicine, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
    3 Department of Traditional Chinese Medicine, Affiliated Hospital of Binzhou Medical College, Shandong 256603, China
Dr. HUANG Shuaiyang, Department of Respiratory Medicine, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China. 1138507954@qq.com. Telephone:+86-10-84013195; +86-18813110509
Prof. WANG Pengwen, Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. pw_wang@163.com;

Received date: 2021-12-12

  Accepted date: 2022-03-26

  Online published: 2023-01-10

Supported by

The Mechanism of Shenzhiling in Early Intervention on AD via Functional Brain Circuit PI3K/Akt-MTOR Pathway(81573927);The Scientific Research and Graduate Training Project of Beijing Municipal Commission of Education (2016), and Young Teachers Project of Beijing University of Chinese Medicine(2019-BUCMXJKY018)

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Abstract

OBJECTIVE: To investigate the efficacy of Jinsiwei (a patented Chinese herbal compound) on learning and memory impairment, the number of synapses and synaptic plasticity-related structural and functional protein expression in mice with sporadic Alzheimer’s disease (SAD) induced by streptozotocin.
METHODS: Seventy-five C57/BL6J male mice were intracerebroventricularly injected with streptozotocin to establish the animal model of SAD. Mice were randomly divided into the model group (MG), donepezil group (DG), and the Jinsiwei high, medium, and low-dose groups (JH, JM, JL). Another fifteen C57/BL6J male mice were injected with artificial cerebrospinal fluid as the control group (CG). The intervention groups were intragastrically administrated with corresponding medicine, while the CG and MG were given 0.5% carboxymethyl cellulose by gavage. After 3 months, the Morris Water Maze test and step-down passive avoidance test were used to assess the learning and memory ability of mice. Synapses in hippocampal CA1 were observed by transmission electron microscope. Immunohistochemistry and western blotting were used to assess the distribution and expression levels of synaptic plasticity-related structural and functional proteins involving drebrin, cofilin, synapsin (syn), and N-methyl D-aspartate receptor subtype 2B (NR2B).
RESULTS: The Morris Water Maze results showed that the escape latency in the Jinsiwei intervention groups was significantly shorter than that of the MG. Results of the step-down passive-avoidance test showed that the error times in the Jinsiwei intervention groups were significantly reduced compared with the MG. Transmission electron microscope results showed that the number of synapses in hippocampal CA1 was obviously increased in the Jinsiwei intervention groups compared with the MG. Immunohistochemical and western blotting results revealed that the positive cells and expression levels of drebrin, syn, and NR2B were significantly decreased in the MG and meanwhile cofilin significantly increased, while these changes were reversed after the Jinsiwei treatment.
CONCLUSIONS: Jinsiwei can alleviate learning and memory impairments in a mouse model of SAD, increase the number of synapses and enhance synaptic plasticity via rescuing the expression of drebrin, syn, and NR2B and inhibiting cofilin expression.

Key words： Alzheimer disease; streptozotocin; neuronal plasticity; Jinsiwei

Cite this article

Meijing TIAN , Yannan HE , Mingcui ZHENG , Gaofeng QIN , Zhuoyan GONG , Shuaiyang HUANG , Pengwen WANG . Chinese herbal compound Jinsiwei (金思维) improves synaptic plasticity in mice with sporadic Alzheimer’s disease induced by streptozotocin[J]. Journal of Traditional Chinese Medicine, 2023 , 43(1) : 78 -86 . DOI: 10.19852/j.cnki.jtcm.20220727.001

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