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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 1: 42 - 50

DOI: https://doi.org/10.19852/j.cnki.jtcm.20221111.001

Original articles

Tilianin extracted from Xiangqinglan (Herba Dracocephali Moldovicae) inhibits apoptosis induced by mitochondrial pathway and endoplasmic reticulum stress in H9c2 cells after oxygen-glucose deprivation/reoxygenation

  • Wen JIANG ,
  • Wei ZHANG ,
  • Yuxiang ZHANG ,
  • Hao YANG ,
  • Xiaomei PAN ,
  • Qiang CHEN ,
  • Junhui CHEN
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  • 1 Department of Pharmacy, the Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China
    2 Department of Anesthesiology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China
    3 Department of Laboratory, the Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China
CHEN Junhui, Department of Pharmacy, the Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China. wensmile1987@126.com. Telephone:+86-9912660042; +86-991-2662027
CHEN Qiang, Department of Pharmacy, the Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China. cq201007@sina.com.

Received date: 2021-12-01

  Accepted date: 2022-02-27

  Online published: 2023-01-10

Supported by

Mechanisms of the Protective Effects of Tilianin Against Myocardium Ischemia/Reperfusion Injury Through Ox-CaMKII-mediated Mitochondrial Regulating Pathway(81760045);Study on the Mechanism of Tilianin Anti-atherosclerosis Effect of Tealis via the Reverse Cholesterol Transport Pathway(2018D01C317);Study on the Mechanism of Tilianin Mediate Mitochondrial Function to Regulate Endoplasmic Reticulum Stress and Participate in Anti-myocardial Ischemia/Reperfusion Injury(320.6750.2020-04-32)

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Abstract

OBJECTIVE: To investigate the efficacy of tilianin extracted from Xiangqinglan (Herba Dracocephali Moldovicae) on apoptosis of H9c2 cell after oxygen-glucose deprivation/reoxygenation (OGD/R) and the mechanism.
METHODS: Tilianin was obtained from Beijing Inluck Science and Technology Development Co. Ltd., with purity ≥ 98%. The OGD/R model was established in H9c2 cells. Flow cytometry detected the mitochondrial membrane potential, apoptosis rates, mitochondrial reactive oxygen species (ROS) and calcium ion concentration. Succinate dehydrogenase (SDH) activity, succinate content and levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1 β (IL-1β) were detected with enzyme-linked immunosorbent assay. Western blot measured protein levels.
RESULTS: Tilianin significantly reduced the apoptotic rates, ROS levels, calcium ion concentration, succinate content, and, levels of TNF-α, IL-6 and IL-1β of OGD/R cells, while significantly increased the membrane potential and SDH activity in mitochondria. Western blot analysis showed that tilianin significantly up-regulated p-Calmodulin-dependent protein kinase Ⅱ and voltage-dependent anion selective channel levels in OGD/R cells, while significantly down-regulated p-protein kinase B, Bcl-2-associated X, and dynamin-related protein 1 levels related to apoptosis in the mitochondrial pathway. Moreover, tilianin significantly up-regulated B-cell lymphoma-2 and mitochondrial protein 2 related to the inhibition of apoptosis. Furthermore, tilianin down-regulated phosphorylated-apoptosis signal-regulated kinase 1, phosphorylated-p38 and C/EBP homologous protein related to endoplasmic reticulum stress.
CONCLUSIONS: Tilianin may inhibit OGD/R-induced H9c2 cell apoptosis mediated by mitochondrial pathway and endoplasmic reticulum stress, thus protecting cardiomyocytes.

Key words： apoptosis; mitochondria; endoplasmic reticulum stress; tilianin; oxygen-glucose deprivation/reoxygenation

Cite this article

Wen JIANG , Wei ZHANG , Yuxiang ZHANG , Hao YANG , Xiaomei PAN , Qiang CHEN , Junhui CHEN . Tilianin extracted from Xiangqinglan (Herba Dracocephali Moldovicae) inhibits apoptosis induced by mitochondrial pathway and endoplasmic reticulum stress in H9c2 cells after oxygen-glucose deprivation/reoxygenation[J]. Journal of Traditional Chinese Medicine, 2023 , 43(1) : 42 -50 . DOI: 10.19852/j.cnki.jtcm.20221111.001

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