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Journal of Traditional Chinese Medicine >

2023 , Vol. 43 >Issue 1: 113 - 123

DOI: https://doi.org/10.19852/j.cnki.jtcm.2023.01.010

Original articles

Efficacy of suspended moxibustion stimulating Shenshu (BL23) and Guanyuan (CV4) on the amygdala-HPA axis in rats with kidney-Yang deficiency symptom pattern induced by hydrocortisone

  • Youjiang MIN ,
  • Haihua YAO ,
  • Zhiqin WANG ,
  • Kaitao LUO ,
  • Jie SUN ,
  • Zheng YUAN ,
  • Huiqi WU ,
  • Lihong CHENG
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  • 1 School of Traditional Chinese Medicine, Nanchang Medical College, Nanchang 330053, China
    2 Traditional Chinese Medicine Department, Shanghai Eighth People’s Hospital, Shanghai 200235, China
    3 Acupuncture Department, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi, China
    4 Acupuncture Department, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing 314001, China
LUO Kaitao, Acupuncture Department, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing 314001, China. lkt740813@163.com. Telephone:+8618217463558; +8613758395805
YAO Haihua, Outpatient Clinic Office, Shanghai Eighth People’s Hospital, Shanghai 200235, China. myj2002@126.com;

Received date: 2021-12-21

  Accepted date: 2022-03-11

  Online published: 2023-01-10

Supported by

Study on the Mechanism of Suspension Moxibustion with Moxa Stick in Treating Hydrocortisone Rats with Kidney-Yang Deficiency based on Hippocampus (amygdala)-HPA Axis(81660817)

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Abstract

OBJECTIVE: To investigated the effects of suspended moxibustion stimulating Shenshu (BL23) and Guanyuan (CV4) acupoints on the amygdala and HPA axis in our rat model and elucidated the possible molecular mechanisms of moxibustion on kidney-Yang deficiency symptom pattern (KYDS).
METHODS: Sixty male Sprague Dawley rats were randomly divided into a control group (n = 12) and an experimental group (n = 48). Rats in the experimental group were given intramuscular injections of hydrocortisone to establish a KYDS model. The 48 rats successfully modeled were then randomly divided into a model group (model, n = 12), a carbenoxolone intraperitoneal injection group (CBX, n = 12), a moxibustion group (moxi, n = 12), and a moxi + CBX group (n = 12). In the moxi, the Shenshu (BL23) and Guanyuan (CV 4) acupoints were treated with moxibustion for 14 d. After treatment, measures were taken of serum levels of corticosterone (CORT), adrenocorticotropic hormone (ACTH), and corticotropin-releasing hormone (CRH). The expression of mineralocorticoid receptors (MRs), glucocorticoid receptors (GRs), 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1), CRH, and ACTH in the rats' amygdala, hypothalamus, or pituitary (as appropriate) was detected. Data were analyzed using one-way analysis of variance.
RESULTS: Compared with those of the control group, the serum levels of CRH, ACTH, and CORT; the mRNA and protein expressions of MR, GR, and 11β-HSD1 in the amygdala; the mRNA and protein expressions of 11β-HSD1 in the hypothalamus; the CRH mRNA expression in the amygdala and hypothalamus; and the ACTH mRNA expression in the pituitary of the rats in the model group were all significantly decreased (P < 0.05 or 0.01). After treatment with moxibustion, all the aforementioned observation indices except for 11β-HSD1 mRNA expression were ameliorated compared with those in the model group (P < 0.05 or 0.01).
CONCLUSIONS: Suspended moxibustion can effectively improve the serum levels of ACTH, CRH, and CORT and can up-regulate the mRNA and protein expressions of MR, GR, 11β-HSD1, CRH, and ACTH in the amygdala and hypothalamus of KYDS rats. This may be one of the molecular mechanisms with which moxibustion alleviates KYDS.

Key words： moxibustion; kidney-Yang deficiency; amygdala; hypothalamus; receptors, mineralocorticoid; receptors, glucocorticoid; 11-beta-hydroxysteroid dehydrogenase type 1

Cite this article

Youjiang MIN , Haihua YAO , Zhiqin WANG , Kaitao LUO , Jie SUN , Zheng YUAN , Huiqi WU , Lihong CHENG . Efficacy of suspended moxibustion stimulating Shenshu (BL23) and Guanyuan (CV4) on the amygdala-HPA axis in rats with kidney-Yang deficiency symptom pattern induced by hydrocortisone[J]. Journal of Traditional Chinese Medicine, 2023 , 43(1) : 113 -123 . DOI: 10.19852/j.cnki.jtcm.2023.01.010

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