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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 6: 940 - 947

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.06.007

Research Articles

Effect of Dangfei Liganning capsule (当飞利肝宁胶囊) on liver X receptor α/steroid regulatory element binding protein-1/fatty acid synthase signal pathway in rats with metabolic- associated fatty liver disease

  • Xiaoling LI ,
  • Fengxia SUN ,
  • Zimeng SHANG ,
  • Yingxue ZHANG ,
  • Jie LI ,
  • Qiuxiang ZHANG
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  • 1 Infections Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China
    2 Infections Department, Beijing Hospital of Traditional Chinese Medicine Shunyi Branch, Beijing 101300, China
Pro. SUN Fengxia, Infections Department, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China. sunfengxia01969@163.com, Telephone: +86-10-87906608

Received date: 2021-08-12

  Accepted date: 2021-11-27

  Online published: 2022-11-01

Supported by

Capital Health Development Research Project: Assessment of the Efficacy of BIEJIAJIANWAN Pill in Patients with Chronic Hepatitis B Cirrhosis/Fibrosis(CD2018-2-2173);Beijing Municipal Administration of Hospitals Incubating Program: Clinical Observation on the Treatment of Nonalcoholic Fatty Liver Disease by Invigorating the Spleen, Soothing the Liver, Activating Blood Circulation and Resolving Phlegm(PZ2019011)

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Abstract

OBJECTIVE: To study the mechanism of Dangfei Liganning capsule (当飞利肝宁胶囊) in the treatment of rats with metabolic associated fatty liver disease (MAFLD).

METHODS: Totally 48 specific pathogen free Sprague-Dawley male rats were randomly divided into normal Group, model group, Dangfei Liganning high, moderate, and low-dose groups and Essentiale group which were fed with high fat diet for 8 weeks, and gavage and molding were carried out simultaneously. Dangfei Liganning high, middle and low-dose group were given 0.27, 0.135 and 0.0675 g·kg-1·d-1 respectively by gavage, Essentiale group was given 0.123 g·kg-1·d-1 by gavage, the same amount of distilled water was given by gavage in the normal group and the model group. The rats were weighed at the 0th week, 2nd week, 4th week, 6th week and 8th weekend respectively. The rats were sacrificed at the end of the 8th week. Serum levels of alanine aminotransferase (ALT), alanine aminotransferase (AST), triglyceride (TG), total cholesterol (CHO), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein (LDL-C), total protein (TP), albumin (Alb), globulin (GLB), total bilirubin (TBIL), direct bilirubin (DBIL), tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) were measured. The levels of liver tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and liver pathology [hematoxylin and eosin (HE) staining, oil red O staining] were detected. The expression levels of liver X receptor α (LXRα), steroid regulatory element binding protein-1 (SREBP-1) and fatty acid synthase (FAS) were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction reverse transcription-polymerase chain reaction.

RESULTS: From the beginning to the 8th week, the growth rate of body weight in the Dangfei Liganning high-dose group was slower than all other groups. There was no significant difference in ALB level in all groups (P > 0.05). Compared with the model group, the levels of ALT, AST, LDL-C, TG, CHO, TP, GLB, TBIL, DBIL, IL-6, TNF-α were significantly decreased and HDL-C were significantly increased in Dangfei Liganning high-dose group (P < 0.01, < 0.05). HE and oil red O staining showed that the fatty lesions in rat liver were alleviated, while the expressions of LXRα, SREBP-1, FAS mRNA and protein were significantly decreased (P < 0.01).

CONCLUSIONS: Dangfei Liganning capsule can slow down the increase of body weight of MAFLD rats, reduce the levels of transaminase, Lipid and inflammatory factors in MAFLD rats, promote the synthesis of liver protein and bile metabolism, and improve the liver fatty lesion of MAFLD rats, among which the Dangfei Liganning high-dose group is more effective. The mechanism of action may be through blocking LXR-SREBP-1-FAS signal pathway.

Key words： metabolic associated fatty liver disease; liver X receptors; steroid regulatory element binding protein-1; fatty acid synthases; signal transduction; Dangfei Liganning capsule.

Cite this article

Xiaoling LI , Fengxia SUN , Zimeng SHANG , Yingxue ZHANG , Jie LI , Qiuxiang ZHANG . Effect of Dangfei Liganning capsule (当飞利肝宁胶囊) on liver X receptor α/steroid regulatory element binding protein-1/fatty acid synthase signal pathway in rats with metabolic- associated fatty liver disease[J]. Journal of Traditional Chinese Medicine, 2022 , 42(6) : 940 -947 . DOI: 10.19852/j.cnki.jtcm.2022.06.007

References

[1] Gao X. New thoughts about renaming nonalcoholic fatty liver disease. Lin Chuang Gan Gan Bing Za Zhi 2020; 36: 1201-4.
[2] Wu J, Peng YZ. Efficacy of Dangfei Liganning capsule in nonalcoholic fatty liver disease. Shi Yong Lin Chuang Yi Xue 2018; 19: 4-6.
[3] Hong HW, Zhao XW, Zhang SM, et al. Clinical observation on Dangfei Liganning Capsule combined with Chinese herbal tea in treating 37 cases of non-alcoholic fatty liver with liver constraint and spleen deficiency and damp-heat accumulation syndrome. J Tradit Chin Med 2018; 59: 227-30.
[4] Alba B, Esther GJ, Jose AP et al. Molecular pathways in nonalcoholic fatty liver disease. Clin Exp Gastroenterol 2014; 7: 221-239.
[5] Liu Y, Qiu de K, Ma X. Liver X receptors bridge hepatic lipid metabolism and inflammation. J Dig Dis 2012; 13: 69-74.
[6] Chen G, Liang G, Ou J, et al. Central role for liver X receptor in insulinmediated activation of Srebp-1c transcription and stimulation of fatty acid synthesis in liver. Proc Natl Acad Sci USA 2004; 101: 11245-50.
[7] Victoria J, Yongyong H, Miwon A, et al. Impact of silencing hepatic SREBP-1 on insulin signaling. PLoS One 2018; 13: e0196704.
[8] Chen LR, Zhang LP, Liu Y, et al. Effects of Chaiqi Tang on expression of SREBP-1c in rats with nonalcoholic fatty liver disease. Xi Bu Zhong Yi Yao 2016; 29: 13-7.
[9] Christian VL, Stefanie L, Frank NR, et al. The human longevity gene homolog INDY and interleukin-6 interact in hepatic lipid metabolism. Hepatology 2017, 66: 616-30.
[10] Nati M, Haddad D, Birkenfeld A L, et al. The role of immune cells in metabolism-related liver inflammation and development of non-alcoholic steatohepatitis (NASH). Rev Endocr Metab Dis 2016; 17: 29-39.
[11] Zhang LP. Efficacy and safety of Tiopronin sodium combined with Shenxiong glucose injection in the treatment of fatty liver. Lin Chuang He Li Yong Yao Za Zhi 2021; 14: 67-9.
[12] Tang WJ, Yao YF, Yin JJ, et al. Effect of Huganqingzhi tablet on protein synthesis and bile metabolism in liver of Steatosis rats. Chinese Materia Medica 2014; 37: 1452-4.
[13] Mitro N, Mak PA, Vargas L, et al. The nuclear receptor LXR is a glucose sensor. Nature 2007; 445: 219-23.
[14] Yan CX, Zhang YR, Zhang XX, et al. Curcumin regulates endogenous and exogenous metabolism via Nrf2-FXR-LXR pathway in NAFLD mice. Biomed. Pharmacother 2018; 105: 274-81.
[15] Pan YX, Zhuo MQ, Li DD, et al. SREBP-1 and LXRα pathways mediated Cu-induced hepatic lipid metabolism in zebrafish Danio rerio. Chemosphere 2019; 215: 370-9.
[16] Yvan G, Nicolas D, Vanessa B, et al. Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues. J Lipid Res 2005; 46: 697-705.
[17] Young AM. The SCAP/SREBP pathway: a mediator of hepatic steatosis. Endocrinol Metab 2017; 32: 6-10.
[18] Shi L J, Shi L, Song GY, et al. Oxymatrine attenuates hepatic steatosis in non-alcoholic fatty liver disease rats fed with high fructose diet through inhibition of sterol regulatory element binding transcription factor 1 (Srebf1) and activation of peroxisome proliferator activated receptor alpha (Pparα). Eur J Pharmacol 2013; 714: 89-95.
[19] Yang ZX, Shen W, Sun H. Effects of nuclear receptor FXR on the regulation of liver lipid metabolism in patients with non-alcoholic fatty liver disease. Hepatol Int 2010; 4: 741-8.
[20] Chen LR. Clinical retrospective study of youth non-alcoholic fatty liver disease and study of the mechanism of Chaiqi decoction on fatty liver in rats. Beijing: Beijing University of Chinese Medicine, 2017: 62-121.
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