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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 6: 908 - 918

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.06.006

Research Articles

Characterization and anticancer potential of Withania somnifera fruit bioactives (a native species to Pakistan) using gas chromatography-mass spectrometer, nuclear magnetic resonance and liquid chromatography-mass spectrometry-electrospray ionization

  • Tabassam Qudsia ,
  • Mehmood Tahir ,
  • Ahmed Sibtain ,
  • Anwar Farooq ,
  • Rauf Raza Abdul
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  • 1 Institute of Chemistry, University of Sargodha, Sargodha-40100-, Pakistan
    2 Institute of Chemistry, University of Sargodha, Sargodha-40100, Pakistan; Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-UVAS, 54000-Lahore. Pakistan
    3 University of California San Diego, 9500 Gilman Drive La Jolla, CA 92093, USA
Sibtain Ahmed, University of California San Diego, 9500 Gilman Drive La Jolla, CA 92093, USA sibtain@ucsd.edu
Tahir Mehmood, Institute of Chemistry, University of Sargodha, Sargodha-40100-, Pakistan; Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-UVAS, 54000-Lahore. Pakistan. tahir.mehmoodbiochem@uvas.edu.pk;

Received date: 2020-11-16

  Accepted date: 2021-01-12

  Online published: 2022-11-01

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Abstract

INTRODUCTION: Withania somnifera (W. somnifera) is a plant with remarkable pharmacological properties. The plant has an impressive profile of medicinal uses in the folk medicine system of several civilizations.

AIM: This comprehensive study is aimed to characterize phytochemicals in fruit of W. somnifera and tested for in vitro anticancer potential to find out active candidate in disease prevention and treatment.

METHODS: The bioactive components from W. somn-ifera fruit were extracted with polar and non-polar solvents. Anticancer potential of the isolated bioactive was assessed against different cancer cell lines through MTT assay and Incucytes imaging analysis. The extracts were characterized for secondary metabolites using GC-MS (gas chromatography-mass spectrometer), LCMS (liquid chromatography-mass spectrometry)-ESI (electrospray Ionization) and 1H-NMR (electrospray Ionization) techniques.

RESULTS: Both freeze-dried and rotary evaporator con-densed extracts exhibited anticancer potential against MDA-MB-231, MCF7- SKOV3 and SKBR3 cell lines. The tested extracts have cell growth inhibition potential ag-ainst mammalian cancer cell line. Hexacosanedioic acid purified from n-hexane extract through HPLC was inves-tigated for its cytotoxicity against breast cancer cell line SKBR3 by using Incucytes imaging analysis. Conclusion: We found that a variety of bioactive compounds existed in this plant. One identified compound that was not investigated for cytotoxicity in previous studies was purified and its application showed cytotoxicity on breast cancer cell lines. A number of bioactive identified from W. somnifera fruit may have an effective potential for development into chemotherapy drugs.

Key words： phytochemicals; plants, medicinal; anti-cancer activities; purification; state-of-the-art techniques

Cite this article

Tabassam Qudsia , Mehmood Tahir , Ahmed Sibtain , Anwar Farooq , Rauf Raza Abdul . Characterization and anticancer potential of Withania somnifera fruit bioactives (a native species to Pakistan) using gas chromatography-mass spectrometer, nuclear magnetic resonance and liquid chromatography-mass spectrometry-electrospray ionization[J]. Journal of Traditional Chinese Medicine, 2022 , 42(6) : 908 -918 . DOI: 10.19852/j.cnki.jtcm.2022.06.006

References

[1] Bhaskar G, Nirbhay K, Abul A, Sunil R. Phytochemical screening of various extracts of root of Withania somnifera (L). Ginseng Res 2018; 42: 463-9.
[2] Kalra R, Kaushik. Withania somnifera (Linn.) Dunal: a review of chemical and pharmacological diversity. Phytochem Review 2017; 16: 953-87.
[3] Gavande K, Jain K, Jain B, Mehta R. Comprehensive Report on Phytochemistry and Pharmacological Prominence of Withania somnifera. UK Pharm Biosci 2015; 3: 15.
[4] Tahir M, Sehrish S, Qudsia T, A. Muhammad A. Saeed. Variation in antioxidant attributes, individual phenolic acids composition and biological activities of Thymus vulgaris: effects of extraction solvents. Int J Biosci 2015; 6: 73-86.
[5] Tabassam Q, Mehmood T, Anwar F, Saari N, Qadir R. Characterization of phenolics and biological activities of different solvent extracts from Withania somnifera fruit. I Food Res J 2020; 27: 915-24.
[6] Tabassam Q, Mehmood T, Raza AR, Ullah A, Saeed F, Anjum FM. Synthesis, Characterization and Anti-Cancer Therapeutic Potential of Withanolide-A with 20nm sAuNPs Conjugates Against SKBR3 Breast Cancer Cell Line. Inter J Nanomedicine 2020; 15: 6649.
[7] Hsu F, Turk J. Studies on sulfatides by quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes that include an unusual internal galactose residue loss and the classical charge-remote fragmentation. J Am Soc Mass Spectrom 2004; 15: 536-46.
[8] Trivedi K, Panda P, Sethi K, Jana S. Liquid chromatography mass spectrometry (LC-MS) analysis of withania somnifera (ashwagandha) root extract treated with the energy of consciousness. A. J Quantum Chem Mol Spectr 2017; 1: 21-30.
[9] Wang Y, Li X, Jiang Q, Mol. GC-MS analysis of the volatile constituents in the leaves of 14 compositae plants. Basel Switz 2018; 23: 166.
[10] Seal T. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves, Sonchus arvensis and Oenanthe linearis of North-Eastern region in India. J Appl Pharm Sci 2016; 1:157-166.
[11] Kang KZ, Vuangho L, Moses E. The in vitro and in vivo anticancer properties of moringa oleifera. Evid Based Compl Alter Med 2018; 1-14.
[12] Lichota A, Gwozdzinski K. Anticancer activity of natural compounds from plant and marine environment. Int J Mol Sci 2018; 19: 3533.
[13] Khazal F, Hill L. Withania somnifera extract reduces the invasiveness of MDA-MB-231 breast cancer and inhibits cytokines associated with metastasis. J Cancer Metastasis Treat 2015; 1: 94-100.
[14] Henley B, Yang L, Chuang L. Withania somnifera root extract enhances chemotherapy through ‘priming. PLoS One 2017; 12.
[15] Grauso L, Emrick S, Bonanomi G, Lanzotti V. Metabolomics of the alimurgic plants Taraxacum officinale, Papaver rhoeas and Urtica dioica by combined NMR and GC-MS analysis. Phytochem Ana 2019; 30: 535.
[16] Krishnan K, Mani A, Jasmine S. Cytotoxic activity of bioactive compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester extracted from a marine derived streptomyces sp. Int J Mol Cell 2014; 3: 246-54.
[17] Vimalavady A, Kadavul K. Phytocomponents identified on the various extracts of stem of Hugonia mystax L. (Linaceae). Euro J Exp Biol 2013; 8.
[18] Faridha BI, Mohankumar R, Jeevan M, Ramani K. GC-MS analysis of bio-active molecules derived from paracoccus pan-totrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROS. Indian J Microbiol 2016; 56: 426-32.
[19] Mericli F, Becer E, Kabaday H. Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil. Pharm Biol 2017; 55: 1239-48.
[20] Nahid R, Ali S, Farshid S. Antimicrobial activity and constituents of the hexane extracts from leaf and stem of Origanum vulgare L. ssp. Viride (Boiss.) Hayek. growing wild in Northwest. Iran. Int J Med Plant Res 2012; 6: 2681-5.
[21] Boussaada O, Saleem A, Saidana D, et al. Chemical composition and antimicrobial activity of volatile components from captula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbiol Res 2008; 163: 87-95.
[22] Xu Y, Qian Y. Anti-cancer Activities of ω-6 Polyunsaturated Fatty Acids. Biomed J 2014; 37: 112-9.
[23] Zhang Q, Su Y, Liu X, Guo Y. Rapid characterization of nonpolar or low-polarity solvent extracts from herbal medicines by solvent-assisted electrospray ionization mass spectrometry. Commun Mass Spectrom RCM 2018; 32: 221-9.
[24] Ben SR, Arafa I, Usam AM. Tentative characterization of polyphenolic compounds in the male flowers of phoenix dactylifera by liquid chromatography coupled with mass spectrometry and DFT. Int J Mol Sci 2017; 18: 512.
[25] Kang J, Price E, Ashton J, Tapsell C, Johnson S. Identification and characterization of phenolic compounds in hydro MeOH extracts of sorghum wholegrains by LC-ESI-MS. Food Chem 2016; 211: 215-26.
[26] Bolleddula J, Fitch W, Vareed K, Nair G. Identification of metabolites in Withania sominfera fruits by liquid chromatography and high-resolution mass spectrometry. Rapid Commun Mass Spectrom 2012; 26: 1277-90.
[27] Hidayat T, Priyandoko D, Wardiny Y, Islami DK. Molecular phylogenetic screening of withania somnifera relative from Indonesia based on internal transcribed spacer region. Hayati J Biosci 2016; 23: 92-5.
[28] Misico I, Nicotra E, Oberti C, Barboza G, Gil R, Burton G. Withanolides and Related Steroids. In: Kinghorn AD, Falk H, Kobayashi J, eds. progress in the chemistry of organic natural products. Springer Vienna 2011; 94:127-229.
[29] Ogungbe V, Setzer N. The potential of secondary metabolites from plants as drugs or leads against protozoan neglected diseases—part iii: in-silico molecular docking investigations. Molecules 2016; 21: 1389.
[30] Pavicic T, Wollenweber U, Farwick M, Korting H. Anti-microbial and -inflammatory activity and efficacy of phytosphingosine: An in vitro and in vivo study addressing acne vulgaris. Int J Cosmet Sci 2007; 29: 181-90.
[31] Braicu C, Pilecki V, Balacescu O, Irimie A, Berindan NI. The relationships between biological activities and structure of flavan-3-ols. Int J Mol Sci 2011; 12: 9342-53.
[32] Trivedi K, Panda P, Sethi K, Jana S. Metabolite Profiling in Withania somnifera roots hydroalcoholic extract using LC/MS, GC/MS and NMR Spectroscopy. Chem Biodivers 2017; 14.
[33] Piasecka A, Kachlicki P, Stobiecki M. Analytical methods for detection of plant metabolomes changes in response to biotic and abiotic stresses. Int J Mol Sci 2019; 20: 379.
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