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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 6: 885 - 891

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.06.004

Research Articles

Buyang Huanwu Tang (补阳还五汤) protects H2O2-induced RGC-5 cell against oxidative stress and apoptosis via reactive oxygen species-mitogen-activated protein kinase signaling pathway

  • Wei ZHENG ,
  • Mingxing WANG ,
  • Shanxue LIU ,
  • Chao LUAN ,
  • Yanqiu ZHANG ,
  • Duoduo XU ,
  • Jian WANG
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  • 1 Department of Ophthalmology, First Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China
    2 Department of Experimental Center, First Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China
    3 Changchun University of Chinese Medicine, Changchun 130117, China
    4 Pharmaceutical Department, First Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China
    5 College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
    6 Department of Encephalopathy Center, First Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China
Prof. WANG Jian, Department of Encephalopathy Center, First Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130021, China. jian-w222@163.com, Telephone: +86-431-86177091

Received date: 2020-11-12

  Accepted date: 2021-02-19

  Online published: 2022-11-01

Supported by

China Postdoctoral Science Foundation (Experimental Study on the Inhibitory Effect of Yiqi Tongluo Method on RGCs Apoptosis in AION Model Rats(2017M621180)

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Abstract

OBJECTIVE: To study the molecular mechanism of Buyang Huanwu Tang(补阳还五汤) (BHT) protecting retinal ganglion cells (RGCs) from oxygen induced oxidative stress and apoptosis after anterior ischemia.

METHODS: In this study, the Chinese herbs of BHT were extracted by first boiling in water, then were filtered, concentrated, and freeze-dried. The chemical profile of BHT extract was determined by liquid chromatography mass spectrometry (LC-MS). H2O2-induced RGC-5 cells were used as a cell model to investigate the protective effect and mechanism of BHT on RGCs.

RESULTS: The survival rate of damaged RGC-5 by BHT was significantly increased by the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazolium-romid method. Fluorescence activating cell sorter (FACS analysis) showed that BHT could significantly reduce apoptosis induced by oxidative stress via the reactive oxygen species (ROS)-mitogen-activated protein kinase (MAPK)-Caspase-3 signal pathway.

CONCLUSION: BHT possesses a high antioxidant capacity and could significantly reduce ROS levels of RGC-5 cells damaged by H2O2.Therefore, the present study has provided possible alternative strategies for the prevention and treatment of ischemic optic disease by using traditional Chinese herbal formulas.

Key words： optic neuropathy, ischemic; oxidative stress; apoptosis; reactive oxygen species; mitogen-activated protein kinase; caspase 3; Buyang Huanwu Tang

Cite this article

Wei ZHENG , Mingxing WANG , Shanxue LIU , Chao LUAN , Yanqiu ZHANG , Duoduo XU , Jian WANG . Buyang Huanwu Tang (补阳还五汤) protects H2O2-induced RGC-5 cell against oxidative stress and apoptosis via reactive oxygen species-mitogen-activated protein kinase signaling pathway[J]. Journal of Traditional Chinese Medicine, 2022 , 42(6) : 885 -891 . DOI: 10.19852/j.cnki.jtcm.2022.06.004

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