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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 781 - 787

DOI: https://doi.org/10.19852/j.cnki.jtcm.20211214.002

Research Articles

Electroacupuncture attenuates chronic salpingitis via transforming growth factor-β1/p38 mitogen-activated protein kinase signaling pathway

  • Xijiao HU ,
  • Yinglong CHENG ,
  • Huanan KANG ,
  • Shuoxi LI ,
  • Yawen WANG ,
  • Jinzhe LIU ,
  • Yiming SUN ,
  • Li LIU
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  • 1 Department of obstetrics and gynecology, the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150001, China
    2 Deapartment of Hysteroscopy chamber, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China
    3 College of pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
    4 Midwifery Teaching and Research Section, Heilongjiang University of Chinese Medicine Jiamusi College, Harbin 150040, China
    5 The department of obstetrics and gynecology, the First Affiliated Hospital of Henan University of Chinese Medicine, Henan 450000, China
    6 Department of Chinese Pharmacy, School of Pharmacy, Guizhou University of Chinese Medicine, Guizhou 550000, China
    7 Andrology department Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150036, China
    8 Endoscopy room, the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Hysteroscopy Department, Harbin 150040, China
Dr. LIU Li, Endoscopy room, the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Hysteroscopy Department, Harbin 150040, China. lunwenfabiao2021@163.com Telephone: +86-15045023666
Dr. SUN Yiming, Andrology department, Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150036, China. sym19660104@163.com.

Received date: 2021-06-12

  Accepted date: 2021-09-21

  Online published: 2021-12-14

Supported by

Scientific Research Project of Heilongjiang Administration of Traditional Chinese Medicine (ZHY18-087): Clinical Study on the Treatment of Tubal Inflammatory Infertility with Gexia Zhuyu Decoction Combined with Hysteroscopic Tubal Fluid Passage;Natural Science Foundation of Heilongjiang Province (H2015026): Study on the Effect of Activating Blood Circulation and Removing Blood Stasis, Activating Qi and Relieving Pain on Salpingitis Infertile Rat Model. Mechanism of Gexia Zhuyu Decoction Improved Fallopian Tube Fibrosis in the Treatment of Salpingitic Obstructive Infertility based on Transforming Growth Factor-Β1/Phosphorylation38 Mitogen-Activated Protein Kinase Pathway(2021ZYGLG001)

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Abstract

OBJECTIVE: To explore the effect of electroacupuncture (EA) on rats with chronic fallopian tube inflammation and its potential mechanisms.

METHODS: Thirty-six female Sprague-Dawley rats were divided into Control, Model and EA groups. The pathological morphology of the fallopian tubes was observed by hematoxylin-eosin (HE) and Masson staining. The results of transforming growth factor-β1 (TGF-β1), P38 mitogen-activated protein kinase (MAPK), phosphorylation (p)-p38MAPK in rat oviduct tissues were detected by immunohistochemistry. Results of P38MAPK, p-P38MAPK and TGF-β1 in rat oviduct tissues were detected by immunofluorescence. The expression level of p38MAPK, p-P38MAPK, TGF-β1 protein in rats was detected by Western blot. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect mRNA expression levels of TGF-β1.

RESULTS: It found that collagen fibers counts decreased significantly in EA group compared to Model group. The phosphorylation of P38MAPK in EA group was significantly reduced compared to Model group. The serum TGF-β1 expressions in EA group increased decreased significantly.

CONCLUSION: Electroacupuncture was able to attenuate chronic salpingitis through down-regulating TGF-β1/MAPK signaling pathway.

Key words： electroacupuncture; salpingitis; transforming growth factor beta1; mitogen-activated protein kinase

Cite this article

Xijiao HU , Yinglong CHENG , Huanan KANG , Shuoxi LI , Yawen WANG , Jinzhe LIU , Yiming SUN , Li LIU . Electroacupuncture attenuates chronic salpingitis via transforming growth factor-β1/p38 mitogen-activated protein kinase signaling pathway[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 781 -787 . DOI: 10.19852/j.cnki.jtcm.20211214.002

References

1. Dun EC, Nezhat CH. Tubal factor infertility: diagnosis and management in the era of assisted reproductive technology. Obstet Gynecol Clin North Am 2012; 39: 551-66.
2. Miller JH, Weinberg RK, Canino NL, Klein NA, Soules MR. The pattern of infertility diagnoses in women of advanced reproductive age. Am J Obstet Gynecol 1999; 181: 952-7.
3 Li Z, Zhang Z, Ming WK, Chen X, Xiao XM. Tracing GFP-labeled WJMSCs in vivo using a chronic salpingitis model: an animal experiment. Stem Cell Res Ther 2017; 8: 272.
4 Liao W, Tang X, Li X, Li T. Therapeutic effect of human umbilical cord mesenchymal stem cells on tubal factor infertility using a chronic salpingitis murine model. Arch Gynecol Obstet 2019; 300: 421-29.
5. Ma K, Wang KL, Chen YX. Infertility caused by salpingitis treated based on theory of kidney deficiency and blood stasis. Zhong Guo Zhong Yao Za Zhi 2019; 44 :1099-103.
6. Stener-Victorin E, Waldenström U, Tägnfors U, Lundeberg T, Lindstedt G, Janson Po. Effects of Electroacupuncture on anovulation in women with polycystic ovary syndrome. Acta Obstet Gyn Scan 2000; 79: 180-8.
7. Mo X, Li D, Pu Y, Xi G, Le X, Fu Z. Clinical studies on the mechanism for acupuncture stimulation of ovulation. J Tradit Chin Med 1993; 13: 115-9.
8. Lyttleton J. Treatment of infertility with Chinese Medicine. 2nd ed. Orlando: Elsevier Health Sciences, 2013: 250-71.
9. Chen BY. Acupuncture normalizes dysfunction of hypothalamic-pituitary-ovarian axis. Acupuncture Electro 1997; 22: 97-108.
10. Chang R, Chung PH, Rosenwaks Z. Role of acupuncture in the treatment of female infertility. Fertil Steril 2002; 78: 1149-153.
11. Titova N, Khashukoeva A, Korneev B. Acupuncture therapy in the comprehensive treatment of salpingo-oophoritis. Akush Ginekol 1991; 44-7.
12. Zhang X, Wei Y, Jiang J, Wang L, Liang H, Lei C. Effect of TGF-β1 on myocardial cell apoptosis in rats with acute myocardial infarction via MAPK signaling pathway. Eur Rev Med Pharmacol Sci 2020; 24: 1350-6.
13. Liang C, Wang S, Qin C, et al. TRIM36, a novel androgen-responsive gene, enhances anti-androgen efficacy against prostate cancer by inhibiting MAPK/ERK signaling pathways. Cell Death Dis 2018; 9: 1-13.
14. Malik S, Suchal K, Khan SI, et al. Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways. Am J Physiol Renal Physiol 2017; 313: F414-22.
15. Wang Q, Zhou C, Zhang D, et al. The involvement of the ERK-MAPK pathway in TGF-β1-mediated connexin43-gap junction formation in chondrocytes. Connect Tissue Res 2019; 60: 477-86.
16. Lv Z, Xu L. Salvianolic acid B inhibits ERK and p38 MAPK signaling in TGF-1-stimulated human hepatic stellate cell line (LX-2) via distinct pathways. Evid-Based Compl Alt 2012; 4: 960128.
17. Bein BN, Zdornov EM. Dynamics of spinal cord motor neuron excitability in epileptic patients following surgical elimination of seizures. Vopr Neirokhir 1976; 1976: 40-4.
18. Aleksandrovych V, Sajewicz M, Walocha JA, Gil K. Tubal telocytes: factor infertility reason? Folia Med Cracov 2016; 56: 17-23.
19. Witkin SS, Toth M, Jeremias J, Ledger WJ. Increased inducibility of inflammatory mediators from peripheral blood mononuclear cells of women with salpingitis. Am J Obstet Gynecol 1991; 165: 719-23.
20. Sikirica V, Bapat B, Candrilli SD, Davis KL, Wilson M, Johns A. The inpatient burden of abdominal and gynecological adhesiolysis in the US. BMC surgery 2011; 11: 13.
21. Clement PB. The pathology of endometriosis: a survey of the many faces of a common disease emphasizing diagnostic pitfalls and unusual and newly appreciated aspects. Adv Anat Pathol 2007; 14: 241-60.
22. Wang Q, Usinger W, Nichols B, et al. Cooperative interaction of CTGF and TGF-β in animal models of fibrotic disease. Fibrogenesis Tissue Repair 2011; 4: 4.
23. Grotendorst GR. Connective tissue growth factor: a mediator of TGF-β action on fibroblasts. Cytokine Growth Factor Rev 1997; 8: 171-79.
24. Li C, Nguyen HT, Zhuang Y, et al. Post-transcriptional up-regulation of miR-21 by type I collagen. Mol Carcinog 2011; 50: 563-70.
25. Coulthard LR, White DE, Jones DL, McDermott MF, Burchill SA. p38MAPK: stress responses from molecular mechanisms to therapeutics. Trends Mol Med 2009; 15: 369-79.
26. Alspach E, Flanagan KC, Luo X, et al. p38MAPK plays a crucial role in stromal-mediated tumorigenesis. Cancer Discov 2014; 4: 716-29.
27. Tsukada S, Westwick JK, Ikejima K, Sato N, Rippe RA. SMAD and p38 MAPK signaling pathways independently regulate alpha1(I) collagen gene expression in unstimulated and transforming growth factor-beta-stimulated hepatic stellate cells. J Biol Chem 2005; 280: 10055-64.
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