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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 749 - 757

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.008

Research Articles

Zebrafish facilitates drug screening: potential of 3-deoxy-andrographoside from Chuanxinlian (Herba Andrographitis Paniculatae) as an anti-inflammatory agent

  • Xuemei HE ,
  • Junjie XIAO ,
  • Chunlin FAN ,
  • Zibin LU ,
  • Huihui CAO ,
  • Linzhong YU ,
  • Yuanru ZHENG ,
  • Junshan LIU
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  • 1 Third Level Research Laboratory of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
    2 Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou 510515, China
    3 Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
    4 School of Pharmacy, Guangdong Pharmaceutical university, Guangzhou 510006, China
    5 Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China
Associate Prof. LIU Junshan, Third Level Research Laboratory of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. liujunshan@smu.edu.cn;

Received date: 2021-05-23

  Accepted date: 2021-08-21

  Online published: 2022-09-02

Supported by

National Science Foundation-funding Project: Study of the Attenuating Effects of 3-dehydroandrographolide on Acute Lung Injury through Modulating Alveolar Macrophage Polarization via Targeting α7nAChR(81973544);Guangzhou Education Bureau University Scientific Research Project: Construction of Traditional Chinese Medicine Discipline(201831845);Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme: Study on the Mechanisms of Anti-Tumor and Anti-Inflammatory Components from Traditional Chinese Medicine based on Detoxification Therapy(GDHVPS2018);Young Elite Scientists Sponsorship Program by CACM: Study on the Mechanisms of Antitumor Components from Traditional Chinese Medicine based on Detoxification Therapy(2019-QNRC2-C14)

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Abstract

OBJECTIVE: To systematically evaluate the in vivo anti-inflammatory potential of diterpene lactones from Chuanxinlian (Herba Andrographitis Paniculatae) (AP).

METHODS: We firstly adopted zebrafish, a novel and ideal animal model for high-throughput drug screening, to investigate the in vivo anti-inflammatory activities of 17 diterpene lactones isolated from AP.

RESULTS: The results showed that most of diterpene lactones displayed significant anti-inflammatory effects in lipopolysaccharide microinjection-, copper sulfate exposure- or tail transection-induced zebrafish inflammation models. Moreover, diterpene lactone 3-deoxy-andrographoside (AP-5) was firstly found to attenuate inflammatory responses, which was closely associated with the myeloid differentiation primary response 88/nuclear factor-kappa B and signal transducer and activator of transcription 3 pathways.

CONCLUSION: Our research sheds light on the inestimable roles of zebrafish in high-throughput drug screening, elucidates the potent inhibitory effects of diterpene lactones against inflammation and indicates that AP-5 may serve as a potential alternative agent for the treatment of inflammatory diseases.

Key words： diterpenes lactones; inflammation; zebrafish; myeloid differentiation factor 88; NF-kappa B; STAT3 transcription factor

Cite this article

Xuemei HE , Junjie XIAO , Chunlin FAN , Zibin LU , Huihui CAO , Linzhong YU , Yuanru ZHENG , Junshan LIU . Zebrafish facilitates drug screening: potential of 3-deoxy-andrographoside from Chuanxinlian (Herba Andrographitis Paniculatae) as an anti-inflammatory agent[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 749 -757 . DOI: 10.19852/j.cnki.jtcm.2022.05.008

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