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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 723 - 731

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.006

Research Articles

Huangqi decoction (黄芪汤) attenuates renal interstitial fibrosis via transforming growth factor-β1/mitogen-activated protein kinase signaling pathways in 5/6 nephrectomy mice

  • Jie ZHAO ,
  • Li WANG ,
  • Ai-li CAO ,
  • Yun-man WANG ,
  • Yang-feng CHI ,
  • Yi WANG ,
  • Hao WANG ,
  • Wen PENG
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  • 1 Department of Chinese Medicine/Department of Chinese Integrative Medicine, The First Affiliated Hospital, Anhui Medical University, Hefei 230022, China
    2 Department of Nephrology, Putuo Hospital affiliated to Shanghai University of Chinese Medicine, Shanghai 200062, China
    3 Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Chinese Medicine, Shanghai 200062, China
Prof. WANG Hao, Department of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Chinese Medicine, Shanghai 200062, China. wang402hao@163.com
Prof. PENG Wen, Department of Nephrology, Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Chinese Medicine, Shanghai 200062, China. pengwen_01@vip.sina.com;

Received date: 2021-07-12

  Accepted date: 2021-10-14

  Online published: 2022-09-02

Supported by

National Natural Science Fund(81903994);Youth Development of the First Affiliated Hospital of Anhui Medical University(2793);Budget Project of Shanghai University of Chinese Medicine(2019LK095)

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Abstract

OBJECTIVE: To investigate the effect of Huangqi decoction (黄芪汤) on renal interstitial fibrosis and its association with the transforming growth factor-β1 (TGF-β1) / mitogen-activated protein kinase (MAPK) signaling pathway.

METHODS: 120 C57/BL mice were randomly divided into six groups: sham group, Enalapril (20 mg/kg) group, 5/6 nephrectomy model group, and 5/6 nephrectomy model plus Huangqicoction (0.12, 0.36 and 1.08 g/kg respectively) groups. Detecting 24hours urinary protein, blood pressure, serum creatinine, urea nitrogen content changes. Periodic Acid-Schiff stain (PAS) and Masson's trichrome staining was used to observe the renal tissue pathological changes. Protein expression of TGF-β1, Phosphorylated P38 mitogen activated protein kinases (P-P38), Phosphorylated c-jun N-terminal kinase (P-JNK), Phosphorylated extracellular regulated proteinhnase (P-ERK), Fibroblast-specific protein-1 (FSP-1), Alpha smooth muscle actin (α-SMA), Type III collagen (Collagen III), Connective tissue growth factor (CTGF), Bcl-2 Assaciated X protein (Bax) and B cell lymphoma 2 (Bcl-2) were measured with western blot and immunohistochemical.

RESULTS: Both Huangqi decoction and Enalapril improved the kidney function, 24 h urinary protein and the fibrosis in 5/6 nephrectomy mice, Huangqi decoction downregulated the expressions of TGF-β1, FSP-1, α-SMA, Collagen III and CTGF in a dose-dependent manner, and it has a significant difference (P < 0.01) compared with model group.Huangqi decoction downregulated the expressions of P-P38, P-JNK, P-ERK and Bcl-2 in a dose-dependent manner, while upregulated the expression of Bax.

CONCLUSIONS: The protective effect of Huangqi decoction for renal interstitial fibrosis in 5/6 nep-hrectomized mice via the inhibition of Epithelial-Mesenchymal Transitions and downregulating the TGF-β1/ MAPK signaling pathway.

Key words： nephrectomy; transforming growth factor beta1; mitogen-activated protein kinases; Signal transduction; renal interstitial fibrosis; Huangqi decoction

Cite this article

Jie ZHAO , Li WANG , Ai-li CAO , Yun-man WANG , Yang-feng CHI , Yi WANG , Hao WANG , Wen PENG . Huangqi decoction (黄芪汤) attenuates renal interstitial fibrosis via transforming growth factor-β1/mitogen-activated protein kinase signaling pathways in 5/6 nephrectomy mice[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 723 -731 . DOI: 10.19852/j.cnki.jtcm.2022.05.006

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