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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 715 - 722

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220707.003

Research Articles

Antihepatofibrotic effect of Guizhifuling pill (桂枝茯苓丸) on carbon tetrachloride-induced liver fibrosis in mice

  • Baogui XU ,
  • Jiawen ZHENG ,
  • Xiaoxiao TIAN ,
  • Falei YUAN ,
  • Zhongliang LIU ,
  • Zuisu YANG ,
  • Xianjun DING
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  • 1 School of Food and Pharmacy, Zhejiang Ocean University; Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, Zhoushan 316022, China
    2 Department of Oncology, Zhoushan Hospital of Traditional Chinese Medicine, Zhoushan 316000, China
    3 Department of Infectious Diseases, Zhoushan Hospital of Traditional Chinese Medicine, Zhoushan 316000, China
Dr. DING Xianjun, Department of Infectious Diseases, Zhoushan Hospital of Traditional Chinese Medicine, Zhoushan 316000, China. d.xj@163.com Telephone: +86-580-2260600
Prof. YANG Zuisu, School of Food and Pharmacy, Zhejiang Ocean University; Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, Zhoushan 316022, China. abc1967@126.com;

Received date: 2021-07-18

  Accepted date: 2021-10-11

  Online published: 2022-07-07

Supported by

Zhejiang Provincial Key Research Project of Traditional Chinese Medicine Science and Technology Plan: Guizhi Fuling Pill Up-regulates MiR-29b-3p and Inhibits CUGBP1-mediated Study on the Mechanism of Liver Fibrosis(2019ZZ017)

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Abstract

OBJECTIVE: To evaluate the protective effects and the underlying mechanism of Guizhifuling pill (桂枝茯苓丸, GZFL) on carbon tetrachloride (CCl4)-induced hepatic fibrosis in mice.

METHODS: Male ICR mice by intraperitoneally administered with 20% CCl4 (mixed 1∶4 in soybean oil) to induce liver fibrosis. Mice that underwent CCl4 were orally with GZFL. Using hematoxylin and eosin and Masson staining to examine the pathological changes in liver tissue. Serum biochemical parameters, antioxidant enzyme activity and proinflammatory cytokines was assessed. Nuclear factor-kappaB (NF-κB) pathway and nuclear factor-erythroid 2-related factor 2 (Nrf2) family members were evaluated by Western blotting.

RESULTS: Our findings indicated that GZFL could effectively suppress the progression of liver fibrosis in mice, which was determined based on the improvement in liver function and reduction of collagen deposition. GZFL treatment also decreased the level of cytokines and increased the activity of antioxidant enzymes in liver tissue. Moreover, GZFL exerted anti-inflammatory and antioxidant effects through regulating the Nrf2-mediated antioxidant system and inhibiting the NF-κB pathway.

CONCLUSIONS: GZFL may prevent the progression of liver fibrosis by regulating the Nrf2/ heme oxygenase-1 and NF-κB signaling pathways, thereby highlighting its role in the management of liver fibrosis.

Key words： liver fibrosis; NF-E2-related factor 2; heme oxygenase-1; NF-kappa B; Guizhifuling pill

Cite this article

Baogui XU , Jiawen ZHENG , Xiaoxiao TIAN , Falei YUAN , Zhongliang LIU , Zuisu YANG , Xianjun DING . Antihepatofibrotic effect of Guizhifuling pill (桂枝茯苓丸) on carbon tetrachloride-induced liver fibrosis in mice[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 715 -722 . DOI: 10.19852/j.cnki.jtcm.20220707.003

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