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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 707 - 714

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.005

Research Articles

Yajieshaba prevents lipopolysaccharide-induced intestinal barrier injury via anti-inflammatory and anti-apoptosis

  • Liping YANG ,
  • Xinglin YU ,
  • Chao ZHANG ,
  • Pu CHEN ,
  • Xiaohua DUAN
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  • Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming 650500, China
DUAN Xiaohua, Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming 650500, China. duanxiaohua@ynutcm.edu.cn Telephone: +86-871-65919481

Received date: 2021-05-22

  Accepted date: 2021-08-23

  Online published: 2022-09-02

Supported by

Grant from the Scientific Research Project of Yunnan Provincial Hospital of Traditional Chinese Medicine: Protective Effect and Mechanism of Yajie Prescription on Intestinal Mucosal Barrier(2015DYYJ101);Yunnan Provincial Science and Technology Department-Applied Basic Research Joint Special Funds of Yunnan University of Traditional Chinese Medicine: Study on the Protective Effect and Mechanism of the Dai Medicine "Yajieshaba" on Intestinal Mucosal Barrier in Food Allergic Mice(2017FF116-026)

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Abstract

OBJECTIVE: To investigate the protective effect of Yajieshaba (YJSB) on the intestinal barrier dysfunction induced by lipopolysaccharide (LPS).

METHODS: C57BL/6 mice and rat intestinal epithelial cells were treated with LPS. Thiazolyl Blue Tetrazolium Bromide assay were used to detect cell viability. D-Lactate, diamine oxidase and myeloperoxidase and cytokines were determined by enzyme-linked immunosorbent assay. Western blot was used to detect apoptosis-related proteins and tight junction (TJ) proteins. Real-time quantitative polymerase chain reaction was used to quantify the levels of mRNA expression of cytokines. Histological analysis was performed by hematoxylin and eosin staining. An immunofluorescence staining assay was performed to determine the expression level of TJ protein.

RESULTS: YJSB increased cell viability and decreased apoptosis, maintained intestinal permeability after LPS-induced. YJSB inhibited LPS-induced decrease of TJ protein expression, pro-inflammatory cytokine levels and neutrophil infiltration.

CONCLUSION: YJSB protect against LPS-induced intestinal barrier dysfunction via anti-inflammatory and anti-apoptosis, suggesting its therapeutic potential against intestinal barrier injury-related diseases.

Key words： inflammation; apoptosis; lipopolysaccharides; Yajieshaba; intestinal barrier function

Cite this article

Liping YANG , Xinglin YU , Chao ZHANG , Pu CHEN , Xiaohua DUAN . Yajieshaba prevents lipopolysaccharide-induced intestinal barrier injury via anti-inflammatory and anti-apoptosis[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 707 -714 . DOI: 10.19852/j.cnki.jtcm.2022.05.005

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