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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 701 - 706

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.004

Research Articles

Tenglong Buzhong granules (藤龙补中颗粒) inhibits the growth of SW620 human colon cancer in vivo

  • Miao LI ,
  • Jialu ZHENG ,
  • Shuangshuang WANG ,
  • Lei CHEN ,
  • Xiao PENG ,
  • Jinfang CHEN ,
  • Hongmei AN ,
  • Bing HU
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  • 1 Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    3 Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    4 Department of Science and Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
Dr. HU Bing, Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. beearhu@shutcm.edu.cn Telephone: +86-21-64385700

Received date: 2021-07-02

  Accepted date: 2021-10-11

  Online published: 2022-09-02

Supported by

Natural Science Foundation of Shanghai Municipality: Tenglong Buzhong Decoction Inhibiting Metastasis of Colorectal Cancer by Regulation of HIF-1 α/LOX/PTK2 Signal Transduction(20ZR1458700);Program from Science and Technology Commission of Shanghai Municipality: Real World Study of Tenglong Buzhong Decoction on Colorectal Cancer Metastasis and Circulating miRNAs(19401933400)

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Abstract

OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction (藤龙补中汤,TBD), i.e Tenglong Buzhong granules (藤龙补中颗粒, TBG), in human SW620 colon cancer.

METHODS: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG (2.56 g/kg, once per day) and/or 5-Fu (104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry.

RESULTS: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anti-cancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3, -8, and -9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a (CDKN1a) and cyclin-dependent kinase inhibitor 2a (CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein (RB) and expression of cyclin E1 (CCNE1) and cyclin-dependent kinases (CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α).

CONCLUSIONS: TBG inhibits SW620 colon cancer growth, induces apoptosis via SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu.

Key words： colonic neoplasms; apoptosis; cellular senescence; signal transduction; angiogenesis; Tenglong Buzhong granules

Cite this article

Miao LI , Jialu ZHENG , Shuangshuang WANG , Lei CHEN , Xiao PENG , Jinfang CHEN , Hongmei AN , Bing HU . Tenglong Buzhong granules (藤龙补中颗粒) inhibits the growth of SW620 human colon cancer in vivo[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 701 -706 . DOI: 10.19852/j.cnki.jtcm.2022.05.004

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