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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 5: 693 - 700

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.05.003

Research Articles

Qilan preparation (芪蓝颗粒) inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells

  • Jiamin DING ,
  • Yifeng XING ,
  • Zuoliang CHEN ,
  • Wanlu CHEN ,
  • Zhongxiong MA ,
  • Yunde XIE ,
  • Lin ZHOU
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  • 1 Department of Oral Mucosal Diseases, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350000, China
    2 School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China
    3 Department of Oral Mucosal Diseases, Xiamen Stomatological Hospital, School of Stomatology, Fujian Medical University, Xiamen 361003, China
    4 Department of Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350001, China
ZHOU Lin, Department of Implantology, Affiliated Stomatological Hospital of Fujian Medical University, Fuzhou 350001, China. zhoulin@fjmu.edu.cnTelephone: +86-591-83754882

Received date: 2021-05-15

  Accepted date: 2021-08-08

  Online published: 2022-09-02

Supported by

Fujian Provincial Healthy and Family Planning Commission Program: Effects of Qilan Preparation on the Expression of miRNA-21 and Its Target Proteins in Human Tongue Squamous Cell Carcinoma Cells(2017-2-38)

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Abstract

OBJECTIVE: The aim of this study was to examine the antitumor effects of Qilan preparation (芪蓝颗粒) on oral squamous cell carcinoma (OSCC) in vitro and to investigate its underlying mechanisms of action.

METHODS: Cell proliferation, cell cycle distribution and apoptosis were examined using cell counting kit-8 (CCK8) and flow cytometry (FCM). The expression of PTEN and PDCD4 were determined by western blot. Changes in miR-21 levels were quantified using TaqMan stem-loop real-time PCR. After miR-21 was transiently transfected into Tca8113 cells using Lipofectamine®3000, cell proliferation, apoptosis and miR-21 and PDCD4 expression levels were measured.

RESULTS: Qilan preparation inhibited Tca8113 cell growth in a dose- and time-dependent manner by inducing apoptosis and cell cycle arrest in S-phase, decreasing miR-21 levels and increasing PTEN and PDCD4 expression. MiR-21 overexpression reversed the Qilan preparation-induced suppression of cell proliferation and induction of apoptosis while also blocking the increase in PDCD4.

CONCLUSIONS: Our study revealed, for the first time, the ability of Qilan preparation to suppress TSCC cell growth and elucidated that Qilan preparation elicits its anti-cancer actions via either the miR-21/PDCD4 or PTEN pathway.

Key words： microRNAs; squamous cell carcinoma of head and neck; apoptosis; cell cycle checkpoints; Qilan preparation

Cite this article

Jiamin DING , Yifeng XING , Zuoliang CHEN , Wanlu CHEN , Zhongxiong MA , Yunde XIE , Lin ZHOU . Qilan preparation (芪蓝颗粒) inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells[J]. Journal of Traditional Chinese Medicine, 2022 , 42(5) : 693 -700 . DOI: 10.19852/j.cnki.jtcm.2022.05.003

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