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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 4: 556 - 564

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220519.004

Research Articles

Efficacy of Qihuang decoction (芪黄煎剂) on enteric nervous system in rats after gastrectomy

  • Qi ZHANG ,
  • Zhou ZHENG ,
  • Long HUANG ,
  • Hui PENG ,
  • Qingsheng YU ,
  • Laiyong WANG
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  • 1 Department of General Surgery, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China
    2 Institute of Chinese Medicine Surgery, Anhui Academy of Traditional Chinese Medicine, Hefei 230031, China
Prof. YU Qingsheng, Department of General Surgery, First Affliated Hospital of Anhui University of Chinese Medicine, Hefei 230031; Institute of Chinese Medicine Surgery, Anhui Academy of Chinese Medicine, Hefei 230031, China. qsy6312@163.com, Telephone: +86-13866141906

Received date: 2021-08-26

  Accepted date: 2021-11-22

  Online published: 2022-07-12

Supported by

Investigate the Effect of Qihuang Decoction on Gastrointestinal Function and its Molecular Mechanism in Rats after Gastrectomy based on ENS-ICC-SMC Network Architecture(2020yfyzc02);Effect of Qihuang Decoction on Intestinal Lymphocyte Homing after Gastrectomy in Rats(81403406);Qihuang Decoction Inhibits Bacterial Migration during Gastrectomy in Rats and Regulates the M1-type Polarization of Macrophages induced by TLR4/NF-κB Signaling Pathway based on lncRNA MALAT1 Sponge Mir-146a(82174160);Effects of Qihuang Decoction on Gastrointestinal Motility in Rats after Gastrectomy and its Molecular Mechanism were Studied based on ENS Framework(gxyq2019035);Investigate the Inhibition of Cryptotanshinone on 5-FU-SGC-7901 Human Gastric Cancer Cells and its Mechanism based on JAK2/STAT3 Signaling Pathway(2008085MH266)

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Abstract

OBJECTIVE: To investigate the influence of Qihuang decoction (芪黄煎剂) on enteric nervous system after gastrectomy in rats. METHODS: The morphology, distribution and number of intestinal neurons in enteric nervous system (ENS) were observed by immunofluorescence labeling and confocal laser scanning microscopy. Reverse transcription-polymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS. RESULTS: The morphology and distribution of enteric neurons in ENS were changed after gastrectomy, and these neurons in Qihuang decoction group were similar with that of sham operation group. The number of ACh and SP positive neurons, mRNA and protein expression of excitatory neurotransmitters (AChE, SP) and receptors (M3R, NK1R) were decreased after gastrectomy. And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein. For vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS), the number of neurons and mRNA and protein expression of inhibitory neurotransmitters (VIP and NOS) and receptors (VIP2R) were increased after gastrectomy. And these rising indexes fall back after the intervention of Qihuang decoction. Besides, the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group. CONCLUSIONS: These experimental results showed that after gastrectomy, early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate, and consequently enhance gastrointestinal motility.

Key words： Gastrectomy; gastrointestinal motility; enteric nervous system; neurotransmitter agents; receptors, neurotransmitter; Qihuang decoction

Cite this article

Qi ZHANG , Zhou ZHENG , Long HUANG , Hui PENG , Qingsheng YU , Laiyong WANG . Efficacy of Qihuang decoction (芪黄煎剂) on enteric nervous system in rats after gastrectomy[J]. Journal of Traditional Chinese Medicine, 2022 , 42(4) : 556 -564 . DOI: 10.19852/j.cnki.jtcm.20220519.004

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