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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 4: 546 - 555

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220516.003

Research Articles

Studying the nature of ascending-descending-floating-sinking of Chinese medicines based on gonadotropin-releasing hormone

  • Yang FU ,
  • Peipei YUAN ,
  • Mengnan ZENG ,
  • Ying HOU ,
  • Liyuan GAO ,
  • Yaxin WEI ,
  • Xiaoke ZHENG ,
  • Weisheng FENG
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  • Department of Pharmacy, Henan University of Chinese Medicine; The Engineering and Technology Center for Chinese Medicine Development of Henan Province; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry, Zhengzhou 450046, China
Prof. ZHENG Xiaoke, Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China. zhengxk.2006@163.com, Telephone: +86-371-60190296

Received date: 2022-01-12

  Accepted date: 2022-04-19

  Online published: 2022-07-12

Supported by

National Key Research and Development Program: the Major Project for Research of the Modernization of TCM(2019YFC1708802);Henan Province high-level personnel special support: “Zhong Yuan One Thousand People Plan”-Zhongyuan Leading Talent(ZYQR201810080);Key Scientific Research Projects of Colleges and Universities of Henan Province(21A360014)

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Abstract

OBJECTIVE: to explore the nature of ascending-descending-floating-sinking of Traditional Chinese Medicine on normal rats using gonadotropin-releasing hormone (GnRh). METHODS: Normal male Sprague-Dawley rats were orally administered six floating Chinese medicines and seven sinking Chinese medicines for 14 d. then, GnRh and relevant indicators were detected. Initially, the different effects of floating and sinking drugs on the body were explored. To verify the effects of floating and sinking drugs on the body, normal rats were orally administered Mahuang (Herba Ephedra Sinica) and Tinglizi (Semen Lepidii Apetali) for 14 d. Then, GnRh antagonists were administered. We observed the changes of relevant indicators and clarified the correlation between GnRh and the laws of floating and sinking. RESULTS: Floating Chinese medicines significantly increased the testicular coefficients; GnRh level in the serum; the protein level of GnRh receptor (GnRhR) in the testis; and the mRNA levels of androgen receptor (AR), small C-terminal domain phosphatase (SCP) 3, SCP2, SYCE1, SMC1B, SMC3, and Rec8 (P < 0.01 or < 0.05). Sinking Chinese medicines did not react similarly, while GnRh antagonists blocked the regulatory effect of Mahuang (Herba Ephedra Sinica) but did not affect Tinglizi (Semen Lepidii Apetali). CONCLUSIONS: GnRh may be closely related to the nature of ascending-descending-floating-sinking of Chinese medicine. Floating Chinese medicines may promote their medicinal properties by regulating GnRH level; however, sinking Chinese medicines did not affect GnRh level.

Key words： gonadotropin-releasing hormone; receptors, LHRH; Mahuang (Herba Ephedra Sinica); Tinglizi (Semen Lepidii Apetali); testis; ascending-descending-floating-sinking.

Cite this article

Yang FU , Peipei YUAN , Mengnan ZENG , Ying HOU , Liyuan GAO , Yaxin WEI , Xiaoke ZHENG , Weisheng FENG . Studying the nature of ascending-descending-floating-sinking of Chinese medicines based on gonadotropin-releasing hormone[J]. Journal of Traditional Chinese Medicine, 2022 , 42(4) : 546 -555 . DOI: 10.19852/j.cnki.jtcm.20220516.003

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