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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 4: 539 - 545

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220617.004

Research Articles

Wenshen Yangxue decoction (温肾养血方) promotes follicular development in aged female mice via stimulation of the silent information regulator 3/forkhead transcription factor O1 3a pathway

  • Liang ZHANG ,
  • Qian HAN ,
  • Yanxiao YI ,
  • Shaoyang JI ,
  • Mingwei XIN
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  • 1 Department of Clinical Laboratory Medicine, Beijing Hospital of Integrated Traditional Chinese and Western Medicine, Beijing 100039, China
    2 Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, China
    3 Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
Prof. JI Shaoyang, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. jishaoyang@ipe.ac.cn, Telephone: +86-13552866767
Prof. XIN Mingwei, Department of Traditional Chinese Medicine Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing 100026, China. xinmingwei@ccmu.edu.cn;

Received date: 2022-01-11

  Accepted date: 2022-04-22

  Online published: 2022-07-12

Supported by

Study on the Effect of Wenshen Yangxue Recipe on Improving the Quality of Oocytes in Aged Female Mice based on Sirt3/FoxO3a Pathway(7192068);Research on the Pathogenesis of Methyl Group Deletion Caused by One Novel Mutation of BRCA2 Gene in Patients from Families at Risk of Hereditary Ovarian Cancer and Breast Cancer(8972444)

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Abstract

OBJECTIVE: To primarily explore the effect and mechanism of Wenshen Yangxue decoction (温肾养血方) in promoting follicular development in elderly female mice. METHODS: Fifty Institute of Cancer Research mice were randomly divided into blank, controlled ovarian hyperstimulation (COH), low-dose Wenshen Yangxue decoction, medium-dose Wenshen Yangxue decoction, and high-dose Wenshen Yangxue decoction groups, with 10 mice in each group. The number of ovulations, number of fertilizations, mitochondrial adenosine triphosphate (ATP) level, and mitochondrial DNA (mtDNA) of oocytes in each group were compared. Reverse transcription-polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression levels of silent information regulator 3 (Sirt3) and forkhead transcription factor O1 3a (FOXO3a). RESULTS: Wenshen Yangxue decoction significantly increased the number of ovulations in mice (P < 0.05) and promoted the formation of fertilized eggs. The ATP level and mtDNA copy number of mice oocytes in the high-dose groups were significantly higher than those in the COH group (P < 0.05). Wenshen Yangxue decoction significantly increased the mRNA and protein levels of Sirt3 and FOXO3a in mouse oocytes. CONCLUSION: Wenshen Yangxue decoction promoted the development of follicles in elderly female mice, increased the number of ovulations and improved fertility. Its mechanism may be related to increased mitochondrial energy metabolism and regulation of the Sirt3/FOXO3a pathway.

Key words： aging; silent information regulator 3; forkhead transcription factors; oocytes; Wenshen Yangxue decoction

Cite this article

Liang ZHANG , Qian HAN , Yanxiao YI , Shaoyang JI , Mingwei XIN . Wenshen Yangxue decoction (温肾养血方) promotes follicular development in aged female mice via stimulation of the silent information regulator 3/forkhead transcription factor O1 3a pathway[J]. Journal of Traditional Chinese Medicine, 2022 , 42(4) : 539 -545 . DOI: 10.19852/j.cnki.jtcm.20220617.004

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