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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 4: 530 - 538

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.04.003

Research Articles

Drug response biomarkers of Pien Tze Huang (片仔癀) treatment for hepatic fibrosis induced by carbon tetrachloride

  • Di ZHANG ,
  • Muyun WEI ,
  • Luan CHEN ,
  • Hao WU ,
  • Ting WANG ,
  • Zhiruo ZHANG ,
  • Ying ZHANG ,
  • Juan YU ,
  • Jinming HUANG ,
  • Jinhang ZHU ,
  • Shengying QIN
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  • 1 School of Life Sciences, Anhui Medical University, Hefei 230032, China
    2 Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200030, China
    3 Fujian Provincial Key Laboratory of Pien Tze Huang Natural Medicine Research and Development, Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd., Zhangzhou 350000, China
Dr. ZHU Jinhang, Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200000, China. zhujinhang@sjtu.edu.cn
Prof. QIN Shengying, Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200000, China. chinsir@sjtu.edu.cn;

Received date: 2021-01-12

  Accepted date: 2021-03-22

  Online published: 2022-07-12

Supported by

the 863 program(2012AA02A515);the 863 program(2012AA021802);National Nature Science Foundation of China(81773818);National Nature Science Foundation of China(81273596);National Nature Science Foundation of China(30900799);National Nature Science Foundation of China(81671326);National Key Research and Development Program(2017YFC0909303);National Key Research and Development Program(2016YFC0905000);National Key Research and Development Program(2016YFC0905002);National Key Research and Development Program(2016YFC1200200);National Key Research and Development Program(2016YFC0906400);4th Three-year Action Plan for Public Health of Shanghai(Project 15GWZK0101);Shanghai Pujiang Program(17PJD020);Shanghai Key Laboratory of Psychotic Disorders(13dz2260500);Natural Science Foundation of Fujian Province(2016J05210);the Anhui Medical University for Scientific Research of BSKY(XJ201607);Anhui Medical University for Scientific Research(2017xkj006)

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Abstract

OBJECTIVE: To explore biomarkers of Pien Tze Huang (片仔癀) that ameliorated the symptoms of hepatic fibrosis. METHODS: Two groups of carbon tetrachloride-induced hepatic fibrosis (HF) mice model were constructed in our study: one group received PZH treatment and another group received no treatment. We performed this study to investigate the role of PZH in the regulation process of hepatic fibrosis. RESULTS: We identified 31 down-regulated and 39 up-regulated miRNAs using small RNA-seq analysis. Combining RNA-Seq data analysis, our study revealed 7 significant target genes (Sp4, Slc2a6, Tln2, Hmga2, Ank3, Pax9, Fgf9). The results of real-time quantitative polymerase chain reaction analysis suggested that the expression level of 6 genes (Sp4, Tln2, Hmga2, Ank3, Pax9, Fgf9) were down-regulated compared to control group. On the other hand, the expression level of Slc2a6 appeared to be up-regulated. The protein mass spectrometry showed that PZH group had lower protein expression of Tln2 compared to control group. CONCLUSION: We identified 7 genes that were significantly related to PZH response in HF mice using multiple conjoint analysis methods. These genes could participate in underlying regulation mechanism of hepatic fibrosis during PZH treatment.

Key words： liver cirrhosis; biomarkers; RNA-Seq; carbon tetrachloride; fibroblast growth factor 9; Pien Tze Huang

Cite this article

Di ZHANG , Muyun WEI , Luan CHEN , Hao WU , Ting WANG , Zhiruo ZHANG , Ying ZHANG , Juan YU , Jinming HUANG , Jinhang ZHU , Shengying QIN . Drug response biomarkers of Pien Tze Huang (片仔癀) treatment for hepatic fibrosis induced by carbon tetrachloride[J]. Journal of Traditional Chinese Medicine, 2022 , 42(4) : 530 -538 . DOI: 10.19852/j.cnki.jtcm.2022.04.003

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