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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 4: 513 - 519

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220516.002

Research Articles

Effect of astragaloside IV on the immunoregulatory function of adipose-derived mesenchymal stem cells from patients with psoriasis vulgaris

  • Xiuping YIN ,
  • Xiaotong ZHANG ,
  • Rongjia ZHU ,
  • Ping SONG
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  • 1 Department of Dermatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
    2 Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy (BZ0381)/Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China
ZHU Rongjia, Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy (BZ0381)/Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China. zrjia2006@126.com, Telephone: +86-13381260786; +86-15210829378
Prof. SONG Ping, Department of Dermatology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. songping@vip.126.com;

Received date: 2021-03-23

  Accepted date: 2021-06-28

  Online published: 2022-07-12

Supported by

Exploring the Immune Mechanism of Kaixuan Jiedu Method in the Treatment of Psoriasis based on the Hypoxia Sensing Pathway Mediating Th17/Treg Imbalance(82074448)

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Abstract

OBJECTIVE: To compare the phenotype and adipogenic and osteogenic differentiation capacities of adipose-derived mesenchymal stem cells (AMSCs) isolated from patients with psoriasis vulgaris and healthy donors, and to explore the effects of astragaloside IV, a Traditional Chinese Medicine, on the immunoregulatory function of AMSCs. METHODS: AMSCs were isolated from human adipose tissue and cultured for three generations in vitro. Cell phenotype and cell cycle analysis were performed by flow cytometry. Adipogenic and osteogenic differentiation of AMSCs was examined by lipid (oil red O) and alkaline phosphatase staining, respectively. Expression of inflammatory mediators was examined by real-time quantitative polymerase chain reaction analysis, and proliferation was quantified using the cell counting kit-8 assay. RESULTS: Expression of CD29, CD44, and CD73 was higher in AMSCs from healthy donors than psoriasis patients, while the reverse was true for expression of CD45, CD31, and HLA-DR. AMSCs from psoriasis patients had a greater ability to undergo adipogenic differentiation than cells from healthy donors, whereas there was no significant difference in osteogenic differentiation between AMSCs from the two sources. Compared with AMSCs from healthy donors, psoriasis patient-derived AMSCs expressed lower levels of the anti-inflammatory cytokines interleukin-10 and trans-forming growth factor-β (TGF-β) and the immune checkpoint ligand programmed cell death 1 ligand 1 (PD-L1) (P < 0.05) and higher levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). Incubation of AMSCs from psoriasis patients with astragaloside IV had no significant effect on pro-liferation but increased the expression of TGF-β and PD-L1 and decreased the expression of IFN-γ and TNF-α. CONCLUSION: AMSCs from patients with psoriasis vulgaris display abnormal proliferation and adipogenesis and an enhanced pro-inflammatory phenotype. These defects were normalized by treatment with astragaloside IV, suggesting that this Traditional Chinese Medicine may be useful for restoring the immunoregulatory function of AMSCs and immune homeostasis in patients with psoriasis vulgaris.

Key words： psoriasis; mesenchymal stem cells; cell proliferation; astragaloside IV; adipogenesis; osteogenesis; immune regulation

Cite this article

Xiuping YIN , Xiaotong ZHANG , Rongjia ZHU , Ping SONG . Effect of astragaloside IV on the immunoregulatory function of adipose-derived mesenchymal stem cells from patients with psoriasis vulgaris[J]. Journal of Traditional Chinese Medicine, 2022 , 42(4) : 513 -519 . DOI: 10.19852/j.cnki.jtcm.20220516.002

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