OBJECTIVE: To study antimicrobial effect of Sodium houttuyfonate(SH) on Staphylococcus epidermidis(SE) and Candida albicans(CA). METHODS: The prepared strain broths(OD600 =0.05) containing SE and CA were firstly used to test the minimal inhibitory concentrations(MICs) of SH, azithromycin(AZM) and fluconazole(FLU) by micro-dilution method. Then the biofilms of SE and CA were matured in 96-well plates, and co-cultured with SH, AZM and FLU for 1, 2 and 3 days to assess the antibiofilm efficacies of the agents with different concentrations by crystal violet staining method. At last, the treated biofilms of SE and CA by 2× MIC agents were observed by scanning electronic microscope. RESULTS: The MICs of SE and CA were 256 and 1024 μg/mL, respectively. After the 1st, 2nd and 3rd day of medications, the suppressions of biofilm were about 60%(P