Advanced Search

Journal of Traditional Chinese Medicine >

2013 , Vol. 33 >Issue 05: 664 - 668

DOI: https://doi.org/10.1016/s0254-6272(14)60039-x

Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields

Expand
  • Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200011, China;Department of Ophthalmology, Xinhua Hospital, Shanghai JiaoTong University School of Med-icine;Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200092, China;Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, China;

Accepted date: 2013-10-15

  Online published: 2013-10-15

Fold

Abstract

OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P

Key words： Puerarin, Matrix metalloproteinase 2, Electromagnetic fields, Sclera, Fibroblast

Cite this article

Huang Zhu, Xiaojing Cai, Xianqun Fan . Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields[J]. Journal of Traditional Chinese Medicine, 2013 , 33(05) : 664 -668 . DOI: 10.1016/s0254-6272(14)60039-x

Options
Outlines

/

〈 〉