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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 3: 417 - 425

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.03.008

Research Article

Efficacy of phospholipid complex of flavonoids from persimmon leaves on atherosclerosis, and possible mechanism

  • Jinpeng CHEN ,
  • Kexia ZHANG ,
  • Yi LIU ,
  • Song JIN ,
  • Xiaohong GAI ,
  • Tao REN ,
  • Chengwang TIAN
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  • 1 State Key Laboratory of Drug Delivery and Pharmacokinetics, Tianjin 300301, China
    2 Tianjin Key Laboratory of TCM quality markers, Tianjin 300301, China
    3 Tianjin Institute of Pharmaceutical Research, Tianjin 300301, China
    4 Tianjin Pharmaceutial Research Institute Co. Ltd., Tianjin 300462, China
    5 Sinopharm Group Tianjin Co. Ltd., Tianjin 300040, China
TIAN Chengwang, State Key Laboratory of Drug Delivery and Pharmacokinetics, Tianjin 300301, China; Tianjin Key Laboratory of TCM quality markers, Tianjin 300301, China; Tianjin Institute of Pharmaceutical Research, Tianjin 300301, China. tiancw@tjipr.com,Telephone: +86-13672157582

Received date: 2021-09-10

  Accepted date: 2021-11-15

  Online published: 2022-05-20

Supported by

National Science and Technology Major Project of China (Research on Evaluation Technology of New Traditional Chinese Medicine Based on Big Data of Classical Prescription)(2019ZX09201005);International Cooperation Project of Traditional Chinese Medicine (China-Germany International Cooperation in Innovative Research and Development of Traditional Chinese Medicine and Botanical Medicine)(0610-2140NF020630)

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Abstract

OBJECTIVE: To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves (PLF-PC) on atherosclerosis, and to study its mechanism behind the action.

METHODS: To clarify the constituents of the flavonoids from persimmon leaves (PLF), an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was established. To enhance the anti-atherosclerotic effect of PLF, a newly emerging approach based on the combination of phospholipid complexation technique was employed. PLF-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy, Powder X-Ray Diffractometry and Scanning electron microscopy. A model of oxidized low-density lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus PLF. The levels of nitric oxide, endothelial nitric oxide synthase, intracellular adhesion molecule-1, reactive oxygen species, superoxide dismutase, tumor necrosis factor-αand nuclear factor-κB were observed via assay kits.

RESULTS: A total of 31 compounds were identified in PLF. PLF-PC showed better anti-atherosclerotic power compared with PLF, moreover, enzyme linked immune-osorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis via antioxidant-related mechanisms.

CONCLUSIONS: Our findings elucidated that PLF-PC significantly enhanced the PLF’s efficacy on atherosclerosis.

Key words： flavonoids; chromatography,high pressure liquid; mass spectrometry; phospholipids; lipoproteins,LDL; endothelial cells

Cite this article

Jinpeng CHEN , Kexia ZHANG , Yi LIU , Song JIN , Xiaohong GAI , Tao REN , Chengwang TIAN . Efficacy of phospholipid complex of flavonoids from persimmon leaves on atherosclerosis, and possible mechanism[J]. Journal of Traditional Chinese Medicine, 2022 , 42(3) : 417 -425 . DOI: 10.19852/j.cnki.jtcm.2022.03.008

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