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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 3: 408 - 416

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.03.007

Research Article

Effcacy-oriented compatibility for Tianma (Rhizoma Gastrodiae), Yanlingcao (Trillium tschonoskii Maxim) and Bingpian (Borneolum Syntheticum) on improving cerebral ischemia stroke by network pharmacology and serum pharmacological methods

  • Zhiyong LI ,
  • Na ZHU ,
  • Jianliang LI ,
  • Liang FENG ,
  • Yanyan JIANG ,
  • Caifeng LI ,
  • Ling LIN ,
  • Xiulan HUANG
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  • 1 Postdoctoral mobile station of environmental biology, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China
    2 Center for Analytical Chemistry of Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
    3 Pharmacological Department, School of Pharmacy, Minzu University of China, Beijing, 100081, China
    4 Aerosol preparation research center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
    5 School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China
    6 Department of Chinese Materia Medica Chemistry, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China
    7 Academician work station, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China
Prof. LI Zhiyong, Postdoctoral Mobile Station of Environmental Biology, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. lizhiyong7899@sina.com,Telephone: +86-871-65916664

Accepted date: 2021-05-21

  Online published: 2022-05-20

Supported by

Natural Science Foundation of China: Study on the Mechanism of Adjusting Yin and Eliminating Toxicity in Tujia Medicine to Treat Vascular Dementia based on Angiogenesis-regulation by Angiogemic Switch and Nrf2/ARE pathway(81473793);Youth Talent Project from Beijing Education Commission: Optimization of Prescription of Tianzhu San, a Tujia medicine, based on Identification and Targeted Separation of Active Ingredients(YETP1293)

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Abstract

OBJECTIVE: To evaluate the compatibility of Tianma (Rhizoma Gastrodiae, TM), Yanlingcao (Trillium tschonoskii Maxim, YLC) and Bingpian (Borneolum Syntheticum, BP), and their efficacy in the treatment of cerebral ischemic stroke.

METHODS: Network pharmacology was used to determine the compatibility of TM, YLC, and BP, and their potential mechanism. The middle cerebral artery occlusion (MCAO) rat model was used to evaluate the curative effect of the six combinations of TM, YLC, and BP (TZB1-TZB6) on cerebral ischemia, by using the weight matching method to form. The potential component changes of TM and YLC in the blood and brains of rats were analyzed using ultra performance liquid chromatography-mass spectrometry. Finally, molecular docking linked the results of animal experiments and network pharmacology, determining the potential component contributors of TM and YLC to treating ischemic stroke.

RESULTS: TZB reduced the cerebral infarct volume and protected the nerve cells in MCAO rats. The components of TM and YLC were also identified in the blood and brain homogenate, and BP can facilitate the entry of the components of TM and YLC into the blood and brain. Diosgenin, pennogenin, and gastrodin induced effective binding activities with adenosine receptor a1.

CONCLUSION: We investigate an approach that improves the means of folk prescription combined with multi technology that maybe promote the transformation of Chinese medicinal prescription into component-based Chinese medicine.

Key words： infarction,middle cerebral artery; brain ischemia; molecular docking simulation; Adenosine receptor a1; network pharmacology

Cite this article

Zhiyong LI , Na ZHU , Jianliang LI , Liang FENG , Yanyan JIANG , Caifeng LI , Ling LIN , Xiulan HUANG . Effcacy-oriented compatibility for Tianma (Rhizoma Gastrodiae), Yanlingcao (Trillium tschonoskii Maxim) and Bingpian (Borneolum Syntheticum) on improving cerebral ischemia stroke by network pharmacology and serum pharmacological methods[J]. Journal of Traditional Chinese Medicine, 2022 , 42(3) : 408 -416 . DOI: 10.19852/j.cnki.jtcm.2022.03.007

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