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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 2: 227 - 233

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220126.001

Research Articles

Effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure

  • Wei WANG ,
  • Qingling LI ,
  • Qiang MA ,
  • Ran XIA ,
  • Bing GAO ,
  • Yi WANG ,
  • Jing WANG
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  • 1 College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China
    2 Graduate school, Anhui University of Chinese Medicine, Hefei 230012, China
    3 College of Acupuncture and Massage, Anhui University of Chinese Medicine, Hefei 230012, China
    4 Xin’an Medical Key Laboratory; Anhui University of Chinese Medicine, Hefei 230012, China
Prof. WANG Jing, Xin’an Medical Key Laboratory; Anhui University of Chinese Medicine, Hefei 230012, China. wangjing2161@126.com, Telephone: +86-13955189703

Received date: 2021-07-22

  Accepted date: 2021-10-14

  Online published: 2022-01-26

Supported by

National Natural Science Foundation of China: Mechanism of Protective Effects of Moxibustion on Regulating mTOR Signaling Pathway and Inhibiting Autophagy in Rats(81574084);Key Research and Development Program of Anhui Province: Mechanism and Clinical Application of Moxibustion in that Treatment of Chronic Heart Failure(202004j07020045);Open Fund Project of Key Laboratory of Xin’an Medical Education Ministry: Effect of Moxibustion on Myocardial Cell Energy Metabolism in Chronic Heart Failure Patients Based on Xin’an Physician's Theory of Strengthening the Foundation and Cultivating the Original(2020xayx07);Key Natural Science Projects of Anhui Provincial Education Department: Mechanism of Moxibustion Against CHF Fibrosis Based on miR-21/PTEN/mTOR Signaling Pathway-mediated Regulation of Autophagy in Myocardial Cells by circPAN3(KJ2021A0570)

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Abstract

OBJECTIVE: To observe the effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index, the expression levels of apoptosis-related proteins cytochrome c (Cyt-C) and apoptosis-inducing factor (AIF) in chronic heart failure (CHF) rats.

METHODS: Sixty-five rats were randomly divided into normal group (n = 10) and model-I group (n = 55). After modeling, CHF rats in model-I group were divided into model group, moxibustion group, benazepril group, moxibustion plus benazepril group (abbreviated as aibei group, the same below), 10 rats in each group. Echocardiogram index was examined by echocardiography. Hemodynamic indices were measured by rat cardiac function meter. Serum B-type brain natriuretic peptide (BNP) was detected by enzyme-linked immunosorbent assay. Myocardial cells apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling staining. Pathological changes of myocardial tissues were observed by hematoxylin and eosin staining. The expression levels of Cyt-C and AIF in myocardial tissues were detected by Western blot.

RESULTS: Compared with normal group, ejection fraction and left ventricular diameter shortening rate in model-Ⅰ group were significantly reduced, myocardial cells of rats in model group exhibited unclear transverse striations, cells swellings and vacuoles, cardiac functions were deteriorated, serum BNP level, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were significantly increased. Compared with model group, myocardial cells of rats in moxibustion group, benazepril group, and aibei group were dyed more evenly, muscle fibers were arranged relatively neatly, cardiac functions were improved, serum BNP level, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were significantly decreased. Compared with aibei group, cardiac functions were worsened, myocardial cells apoptosis index, and the expression levels of Cyt-C and AIF were increased.

CONCLUSION: Moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril could improve CHF better than moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) or benazepril alone. The mechanisms might be that they can inhibit the expressions of Cyt-C and AIF, and inhibit the apoptosis of cardiomyocytes.

Key words： heart failure; moxibustion; point BL13 (Feishu); point BL15 (Xinshu); cytochromes c; apoptosis inducing factor

Cite this article

Wei WANG , Qingling LI , Qiang MA , Ran XIA , Bing GAO , Yi WANG , Jing WANG . Effects of moxibustion at bilateral Feishu (BL13) and Xinshu (BL15) combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure[J]. Journal of Traditional Chinese Medicine, 2022 , 42(2) : 227 -233 . DOI: 10.19852/j.cnki.jtcm.20220126.001

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