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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 2: 213 - 220

DOI: https://doi.org/10.19852/j.cnki.jtcm.2022.02.003

Research Articles

Modified Gexiazhuyu decoction (膈下逐瘀汤加减方) alleviates chronic salpingitis via p38 signaling pathway

  • Xijiao HU ,
  • Shuoxi LI ,
  • Dongxia YANG ,
  • Na GU ,
  • Jinzhe LIU ,
  • Yawen WANG ,
  • Li LIU ,
  • Yiming SUN
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  • 1 Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150001, China
    2 Jiamusi College of Heilongjiang University of Traditional Chinese Medicine, Heilongjiang 154000, China
    3 Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150001, China
    4 General Hospital of Heilongjiang Forest Industry, Heilongjiang 150040, China
    5 Heilongjiang University of Traditional Chinese Medicine, Heilongjiang 150040, China
    6 First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China
    7 Heilongjiang Academy of Chinese Medicine Sciences, Heilongjiang 150036, China
LIU Li, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang 150040, China. Liuliyouxiang2008@163.com
SUN Yiming, Heilongjiang Academy of Chinese Medicine Sciences, Heilongjiang 150036, China. sym19660104@163.com;

Received date: 2021-04-23

  Accepted date: 2021-07-21

  Online published: 2022-04-01

Supported by

Scientific Research Project of Heilongjiang Administration of Traditional Chinese Medicine: Clinical Study on the Treatment of Tubal Inflammatory Infertility with Gexia Zhuyu Decoction Combined with Hysteroscopic Tubal Fluid Passage(ZHY18-087);Natural Science Foundation of Heilongjiang Province: Study on the Effect of "Activating Blood Circulation and Removing Stasis, Activating Qi and Relieving Pain" on Salpingitis and Infertility Rat Model(H2015026)

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Abstract

OBJECTIVE: To investigate pharmacodynamic effects of modified Gexiazhuyu decoction (MGXZYD) and explore the underlying mechanism in the treatment of chronic salpingitis

METHODS: Chronic salpingitis model rats were firstly constructed and the blood was collected to detect the whole blood viscosity and plasma viscosity. Rat oviduct were collected to evaluate the macroscopic damage and the pathological injury and fibrosis of oviduct by hematoxylin-eosin (HE) and Masson staining. Elisa assay was to detect the production interleukin-1 β (IL-1β) in serum and collagen I (COL-1), matrix metalloprotein 9 (MMP-9), tissue inhibitor of metalloproteinases 1 (TIMP-1) in oviduct tissue. And immunohistochemical staining with MMP-9 and TIMP-1 in oviduct tissue were examined. Western blot was used to detect the expressions of p38 mitogen-activated protein kinases (p38MAPK), phospho-p38MPAK (p-p38MPAK), transforming growth factor-β1 (TGF-β1) in oviduct. The expression of α-smooth muscle actin (α-SMA), p-p38MPAK, in oviduct tissue were detected by immunofluorescence method. The mRNA of p-p38MAPK, α -SMA, COL-1, MMP-9, TIMP-1 was measured by reverse transcription-polymerase chain reaction.

RESULTS: Rats administrated with MGXZYD demonstrated decreased the whole blood viscosity and plasma viscosity. MGXZYD obviously improved the tubal wall thickening, swelling and pelvic adhesion. And HE and Masson staining showed MGXZYD improved the pathological injury and fibrosis of oviduct. The results of MTT assay and flow cytometry indicated that MGXZYD could decreased the NIN-3T3 cells viability and improved the apoptosis. Besides, MGXZYD inhibited the protein and / or mRNA of TGF-β1, IL-1β, COL-1, α-SMA, p-p38MAPK expressions and increased the production of MMP-9/TIMP-1.

CONCLUSION: MGXZYD could prevent the progression of chronic salpingitis by inhibited the fibrocyte and inflammation which via inhibited the p38 MAPK signaling pathway.

Key words： salpingitis; fibrosis; p38 mitogen-activated protein kinases; inflammation; modified Gexiazhuyu decoction

Cite this article

Xijiao HU , Shuoxi LI , Dongxia YANG , Na GU , Jinzhe LIU , Yawen WANG , Li LIU , Yiming SUN . Modified Gexiazhuyu decoction (膈下逐瘀汤加减方) alleviates chronic salpingitis via p38 signaling pathway[J]. Journal of Traditional Chinese Medicine, 2022 , 42(2) : 213 -220 . DOI: 10.19852/j.cnki.jtcm.2022.02.003

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