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Journal of Traditional Chinese Medicine >

2022 , Vol. 42 >Issue 2: 194 - 199

DOI: https://doi.org/10.19852/j.cnki.jtcm.20220311.005

Research Articles

Effect of manipulation on cartilage in rats with knee osteoarthritis based on the Rho-associated protein kinase/LIM kinase 1/Cofilin signaling pathways

  • Xiao GUO ,
  • Yunhao YANG ,
  • Dongmei LIAO ,
  • Fang PANG ,
  • Zhixue YANG ,
  • Zhengwei ZHU ,
  • Ao LUO ,
  • Chenglin TANG
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  • College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400010, China Beibei Traditional Chinese Medicine Hospital, Chongqing 400700, China
Prof. TANG Chenglin, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400010, China. tangchenglin@cqmu.edu.cn, Telephone: +86-23-65712060

Received date: 2021-10-19

  Accepted date: 2022-01-05

  Online published: 2022-03-11

Supported by

National Natural Science Foundation of China(81273870);Chongqing Municipal Health and Family Planning Commission and Chongqing Municipal Science and Technology Commission Jointly Funded Key Research Projects in Traditional Chinese Medicine(ZY201801007);Beibei District Chongqing Basic Research and Frontier Exploration Project(2019-6)

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Abstract

OBJECTIVE: To investigate the effect of manipulation treatment on knee osteoarthritis rats and the effect on Rho-associated protein kinase (ROCK)/LIM-kinase1 (LIMK1)/Cofilin signaling pathway.

METHOD: Fifty Specific pathogen Free Sprague-Dawley rats were randomly divided into five groups (n = 8 each): blank group, model group, manipulation group, celecoxib group, and manipulation combined with celecoxib group (MC group). The osteoarthritis model was established by injecting 0.2 mL 4% papain into the articular disc of the rats. After successfully establishing the model, we treated the manipulation group with pushing manipulation using one-finger-meditation to the Neixiyan (EX-LE4), Waixiyan (EX-LE5), Xuehai (SP10), Liangqiu (ST34), and Zusanli (ST36) acupoints for 10 min each time. Also, the celecoxib group was gavaged with 24 mg•kg-1•d-1 celecoxib, while the MC group was treated using both of these two methods. After four weeks, the cartilage of the right femur was removed for hematoxylin-eosin staining of the cartilage tissue. The expressions of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were observed using the enzyme-linked immunosorbent assay. Besides, we detected the expressions of ROCK, LIMK1, Phospho-LIM-kinase1 (Phospho-LIMK1), Cofilin, and Phospho-Cofilin by Western blot.

RESULTS: Compared to the model group, the manipulation group, celecoxib group, and MC group all exhibited superior results concerning pathological morphologic changes of cartilage, as observed by hematoxylin-eosin staining and calculated using the Mankin score. Besides, in contrast to the blank group, the model group exhibited elevated serum levels of IL-1β and TNF-α (P < 0.01), while the expression of ROCK, LIMK1, Phospho-LIMK1, Cofilin, and Phospho-Cofilin in cartilage were all higher (P < 0.01). Also, the serum levels of IL-1β and TNF-α in each treatment group were lower (P < 0.01) than in the model group. Moreover, there were lower expressions of ROCK, LIMK1, Phospho-LIMK1, Cofilin, and Phospho-Cofilin in cartilage in the manipulation group and the MC group (P < 0.01). Compared with the model group, the expression of ROCK, LIMK1, Phospho-LIMK1, Cofilin, and Phospho-Cofilin in cartilage in the celecoxib group were not statistically different (P > 0.05).

CONCLUSION: In this study, we established that manipulation has a better curative effect than celecoxib. Manipulation inhibits the development of cytoskeleton damage in cartilage and slows articular degeneration by regulating the expression of related proteins in the cytoskeletal signaling pathway.

Key words： osteoarthritis, knee; manipulation; cytoskeletal proteins; Rho-associated kinases; LIM kinases; actin depoly-merizing factors; signal transduction

Cite this article

Xiao GUO , Yunhao YANG , Dongmei LIAO , Fang PANG , Zhixue YANG , Zhengwei ZHU , Ao LUO , Chenglin TANG . Effect of manipulation on cartilage in rats with knee osteoarthritis based on the Rho-associated protein kinase/LIM kinase 1/Cofilin signaling pathways[J]. Journal of Traditional Chinese Medicine, 2022 , 42(2) : 194 -199 . DOI: 10.19852/j.cnki.jtcm.20220311.005

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