OBJECTIVE: To investigate the influence of heated moxa sticks( "moxibustion") and Daiwenjiu ointment(DO) on changes in temperature and blood perfusion volume on the skin of the backs of healthy subjects.METHODS: DO was spread on the left side of the body, and the right side of the body was treated with a heated moxa stick. Images denoting blood perfusion and body temperature were collected 7-8 cm lateral to the spinous process of the sixth thoracic vertebra using laser speckle contrast imaging(LSCI). Data obtained from eight-frame images were analyzed and used to calculate the mean blood perfusion volume. Simultaneously,blood-perfusion images were collected from the body surface and used to compare the change in blood flow on the body surface and the actual position of imaging.RESULTS: After moxibustion, a rapid increase in blood perfusion volume and body temperature was noted in the local skin surface. The maximum blood perfusion volume and body temperature was noted at 20 min(P