OBJECTIVE: To investigate how electroacupuncture(EA) at Zusanli(ST 36) with varying intensities of stimulation affects dorsal root nerve signals in normal rats.METHODS: Adult female Wistar rats were examined after drug-induced anesthesia and isolation of the L4 dorsal root associated with the Zusanli(ST36) acupoint, using bipolar platinum electrodes.We applied EA at Zusanli(ST 36) with a continuous wave waveform for 1 min, and recorded action potentials in the L4 spinal cord dorsal root nerve with a frequency of 5 Hz and various current intensity levels(approximately 1-4 mA).In addition, we tested EA with a frequency of 15 Hz and current intensity of 1-4 mA.The L4 spinal cord dorsal nerve bundle action potentials were measured for 1 min of EA.To analyze the discharge frequency, we used the maximum Lyapunov exponent and Lempel-Ziv(LZ) complexity.RESULTS: At a fixed frequency, with increasing intensity of EA, dorsal root nerve filament discharge frequency revealed an initial increase, followed by a decreasing trend.A stimulation intensity of 3 mA induced a significantly greater discharge frequency, compared with stimulation intensities of 2 and 4 mA.EA stimulation evoked neuroelectric signals with chaotic characteristics.Increased intensity led to an initial increase in LZ complexity which then decreased, with a stimulation strength of 3 mA inducing the highest level of LZ complexity.CONCLUSION: EA of different intensities can induce nerve action potential encoding with different features.