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Journal of Traditional Chinese Medicine >

2019 , Vol. 39 >Issue 03: 356 - 363

DOI: https://doi.org/10.19852/j.cnki.jtcm.2019.03.009

Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease

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  • Department of Mechanics and Engineering Science, Fudan University,Shanghai;Shanghai Research Institute of Acupuncture and Meridian;Department of Acupuncture and Moxibustion,Huashan Hospital,Shanghai;Department of Acupuncture and Moxibustion and Tuina, Guanghua Hospital,Shanghai;

Online published: 2019-06-15

Supported by

Supported by the National Basic Research Program of China(973 Program,No.2015CB554501), Program of National Natural Science Foundation of China(No.81574079), Program for outstanding medical academic leader(2015,No.80), Shuguang Program of Shanghai Education Commission(No.14SG39), Shanghai Rising-Star Program(No.16QA1403400)

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Abstract

OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu(ST 25)and Qihai(CV 6)acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine(DA)and dopamine receptor 1(D1R)in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model(CD),herb-partitioned moxibustion(Mox)and mesalazine(Mesa)groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1R protein expressions and D1R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu(ST 25)and Qihai(CV 6)acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.

Key words： Crohn disease, Dopamine, Receptors,dopamine, Neuropeptides, Point ST25(Tianshu), Point CV6(Qihai), Herb-partitioned moxibustion

Cite this article

Lu Yuan, Zheng Handan, Lü Tingting, Ma Zhe, Wu Huangan, Xu Manwen, Weng Zhijun, Feng Hui, Zhang Fang, Ding Guanghong, Wu Luyi, Liu Huirong . Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease[J]. Journal of Traditional Chinese Medicine, 2019 , 39(03) : 356 -363 . DOI: 10.19852/j.cnki.jtcm.2019.03.009

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