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Journal of Traditional Chinese Medicine >

2020 , Vol. 40 >Issue 3: 393 - 400

DOI: https://doi.org/10.19852/j.cnki.jtcm.2020.03.006

Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro

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  • College of Biology and Environmental Sciences, Jishou University;Research Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences & Peking Union Medical College;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education;IMPLAD, Chinese Academy of Medical Sciences & Peking Union Medical College;College of veterinary medicine, China Agricultural University;Beijing Radiation Center;Key Laboratory of Plant Resources Conservation and Utilization, College of Hunan Province, Jishou University;Experiment Research Center of Xiyuan Hospital, China Academy of Chinese Medical Sciences;

Online published: 2020-06-15

Supported by

Supported by The Special Project for Academic Construction of Peking Union Medical CollegeBeijing (Traditional Chinese Medicine Cultural Construction for Heritage in Chinese Union of Medicinal Plant Gardens,Tsinghua,211-201920100902), Beijing Natural Science Foundation(The molecular mechanism of the retinal pericyte protection by Jangtang Xiaozhi Tablets in STZ induced diabetic rats,No. 7152100)

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Abstract

OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in vitro.METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2'-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability; β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C.CONCLUSION: JXR extract has significant antioxidant capacity in vitro.

Key words： Free radical scavengers, Lipid peroxidation, Antioxidant capacity, Jiangtang Xiaozhi recipe

Cite this article

Song Wang, Yuan Yuan, Yu Naixin, Gu Haike, Zhou Yunfeng, Chen Xigong, Wang Shuaiyu, Fan Kai, Ge Zhengyan, Jin Long, Xu Yang . Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro[J]. Journal of Traditional Chinese Medicine, 2020 , 40(3) : 393 -400 . DOI: 10.19852/j.cnki.jtcm.2020.03.006

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