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Journal of Traditional Chinese Medicine ›› 2013, Vol. 33 ›› Issue (06): 798-803.DOI: 10.1016/s0254-6272(14)60015-7

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Antimicrobial effect of sodium houttuyfonate on Staphylococcus epidermidis and Candida albicans biofilms

Jing Shao, Huijuan Cheng, Daqiang Wu, Changzhong Wang, Lingling Zhu, Zhenxin Sun, Qiangjun Duan, Weifeng Huang, Jinliang Huang   

  1. Laboratory of Microbiology and Immunology, School of Chinese and Western Integrative Medicine, Anhui University of Chinese Medicine;
  • Accepted:2013-12-15 Online:2013-12-15 Published:2013-12-15

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Abstract: OBJECTIVE: To study antimicrobial effect of Sodium houttuyfonate(SH) on Staphylococcus epidermidis(SE) and Candida albicans(CA). METHODS: The prepared strain broths(OD600 =0.05) containing SE and CA were firstly used to test the minimal inhibitory concentrations(MICs) of SH, azithromycin(AZM) and fluconazole(FLU) by micro-dilution method. Then the biofilms of SE and CA were matured in 96-well plates, and co-cultured with SH, AZM and FLU for 1, 2 and 3 days to assess the antibiofilm efficacies of the agents with different concentrations by crystal violet staining method. At last, the treated biofilms of SE and CA by 2× MIC agents were observed by scanning electronic microscope. RESULTS: The MICs of SE and CA were 256 and 1024 μg/mL, respectively. After the 1st, 2nd and 3rd day of medications, the suppressions of biofilm were about 60%(P

Key words: Biofilms, Candida albicans, Drugs,Chinese herbal, Sodium houttuyfonate, Staphylococcus epidermidis

Cite this article

Jing Shao, Huijuan Cheng, Daqiang Wu, Changzhong Wang, Lingling Zhu, Zhenxin Sun, Qiangjun Duan, Weifeng Huang, Jinliang Huang. Antimicrobial effect of sodium houttuyfonate on Staphylococcus epidermidis and Candida albicans biofilms[J]. Journal of Traditional Chinese Medicine, 2013, 33(06): 798-803.