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Journal of Traditional Chinese Medicine ›› 2013, Vol. 33 ›› Issue (05): 664-668.DOI: 10.1016/s0254-6272(14)60039-x

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Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields

Huang Zhu, Xiaojing Cai, Xianqun Fan   

  1. Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200011, China;Department of Ophthalmology, Xinhua Hospital, Shanghai JiaoTong University School of Med-icine;Department of Ophthalmology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200092, China;Department of Ophthalmology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200011, China;
  • Accepted:2013-10-15 Online:2013-10-15 Published:2013-10-15

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Abstract: OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P

Key words: Puerarin, Matrix metalloproteinase 2, Electromagnetic fields, Sclera, Fibroblast

Cite this article

Huang Zhu, Xiaojing Cai, Xianqun Fan. Effect of puerarin on matrix metalloproteinase-2 in human fetal scleral fibroblasts treated with low frequency electromagnetic fields[J]. Journal of Traditional Chinese Medicine, 2013, 33(05): 664-668.