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Journal of Traditional Chinese Medicine ›› 2012, Vol. 32 ›› Issue (03): 453-458.DOI: 10.1016/s0254-6272(13)60054-0

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Amomum villosum induces longitudinal bone growth in adolescent female rats

Lee, Sun Haeng; Kim, Ji Young; Kim, Hocheol; Park, Seul Ki; Kim, Cho Young; Chung, Sun Yong; Chang, Gyu Tae   

  1. Kyung Hee Univ Hosp Gang Dong, Dept Pediat Korean Med, Seoul 134727, South Korea; Kyung Hee Univ, Coll Korean Med, Dept Herbal Pharmacol, Seoul 130701, South Korea; Kyung Hee Univ Hosp Gang Dong, Dept Neuropsychiat Korean Med, Seoul 134727, South Korea
  • Accepted:2012-09-15 Online:2012-09-15 Published:2012-09-15

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Abstract: OBJECTIVE:This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth.METHODS:Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days:control(distilled water,p.o.),recombinant human growth hormone(rhGH;100 μg/kg,s.c.),and A.villosum(500 mg/kg,p.o.) groups.On day 3,tetracycline(20 g/kg,i.p.) was injected for growth plate identification.On days 2,3 and 4,5-bromo-2'-deoxyuridine(BrdU)(50 mg/kg,i.p.) was injected to label proliferating cells.On day 5,tibias were dissected and fixed in 4% paraformaldehyde,dehydrated,and sectioned for immunohistochemistry and histomorphometry.RESULTS:The rate of bone growth in the A.villosum and rhGH groups increased to(410 ± 44) and(389 ± 46) μm/day(P

Key words: Amomum villosum, Growth plate, Bone Development, Recombinant human growth hormone

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Lee, Sun Haeng; Kim, Ji Young; Kim, Hocheol; Park, Seul Ki; Kim, Cho Young; Chung, Sun Yong; Chang, Gyu Tae. Amomum villosum induces longitudinal bone growth in adolescent female rats[J]. Journal of Traditional Chinese Medicine, 2012, 32(03): 453-458.