Abstract: OBJECTIVE: To study the effect of Qilongtoutong granule(QLTT) on plasma calcitonin gene-related peptide(CGRP), beta-endorphin(β-EP), 5-HT, dopamine(DA), noradrenalin(NE), and blood viscosity in migraine model rats and mice.METHODS: Both the acute blood stasis model group and nitroglycerin-induced migraine model group included 60 Sprague-Dawley rats. The reserpine-reduced model group had 60 Kunming mice.Rats from each test were grouped into normal control group, model group, Zhengtian pill(ZTP)group, and high, moderate, or low-dose QLTT groups. In the acute blood stasis model test, after gavage for 7 days, rats were given 0.8 mL/kg adrenaline hydrochloride subcutaneously twice, and kept in ice water for 5 min. After fasting for 12 h,rats were anesthetized and blood samples were col-lected for detection of blood viscosity. In the nitroglycerin-induced migraine group, after gavage for7 days, rats were intraperitoneally injected nitroglycerin(10 mg/kg), and 4 h later, blood samples were collected from postcava for measuring the plasma CGRP and β-EP levels. In the reserpine-reduced model test, except the normal control group, mice were administered reserpine(0.25 mg/kg, i.h.) for 9 days. Mice received intragastric administration from the third day to the ninth day. One hour after the last gavage, blood and brain tissue samples were obtained. Then, blood clotting time and the contents of neurotransmitters were determined.RESULTS: QLTT-(3.6, 1.8, and 0.9 g/kg) and ZTP-treated rats had lower blood viscosity than that in model rats under different shear rates(P

Key words: Migraine disorders, Calcitonin gene-related peptide, Beta-endorphin, Medicine,Chinese traditional, Qilongtoutong granule