Abstract: OBJECTIVE:To investigate the neuroprotective effects of Fructus Chebulae extract using both in vivo and invitromodels of cerebral ischemia.METHODS:As an in vitro model,oxygen glucose deprivation followed by reoxygenation(OGD-R)and hydrogen peroxide(H2O2)induced cellular damage in rat pheochromocytoma(PC12)cells was used to investigate the neuroprotective effects of extract of Fructus Chebulae.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to calculate cell survival.For in vivo,occlusion of left middle cerebral artery on rats was carried out as a focal cerebral ischemic model.RESULTS:Fructus Chebulae extract increases the PC12 cell survival against OGD-R and H2O2by 68%and 91.4%respectively.Fructus Chebulae also de-creases the cerebral infarct volume by 39%and extent of hemisphere swelling from 17%in control group to 10%in FructusChebulaetreated group.CONCLUSION:Fructus Chebulae,as a traditional medicine,can rescue the neuronal cell death against ischemia related damage.The possible mechanism for the neuroprotection might be the inhibition of oxidative damages occurring after acute phase of cerebral ischemia.

Key words: Fructus Chebulae, Pheochromocytoma, Neuroprotection, Brain ischemia, Middle cerebral artery