Abstract: OBJECTIVE: To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa Pakistan.METHODS: Anticancer activity against human lung carcinoma(LU-1) and human prostate carcinoma(Ln Cap), antimicrobial against Aspergillus fumigates, Aspergillus niger, Fusarium solani, Mucor sp Klebsiella pneumonia and Enterococcus and antioxidant activity on 1,1-Diphenyl-2-picryl-hydrazy(DPPH) and Hydrogen peroxide radicals scavenging activity were assessed in methanol extract o Lespedeza bicolor.RESULTS: Lespedeza bicolor aerial parts and root extract showed IC_(50)value of 12.5 and 50 μg/m L against LU-1 whereas, ≤ 12.5 and 12 μg/m L were calculated against Ln Cap cell line. Minimum inhibition concentration(MIC) value of 20-35 μg/m L has been observed against Aspergillus fumigates, Aspergillus niger, Fusarium solani and Mucor sp in comparision with 1-2.5 μg/m L of Terbinafine used as a standard fungicide. MIC value of 20 and 35 μg/m L of Lespedeza bicolor aerial parts and root extract against bacterial pathogen Klebsiella pneumonia and 20-50 μg/m L against Enterococcus has been measured. DPPH radical scavenging activity of Lespedeza bicolor with IC_(50)values of ≤ 50 μg/m L and ≤ 200 μg/m L was observed whereas, hydrogen peroxide scavenging activity with IC_(50)values of ≤25 μg/m L for aerial parts and ≤ 50 μg/m L for the root extract of Lespedeza bicolor has been shown.CONCLUSION: These data suggested that the methanolic extract of Lespedeza bicolor could be potential candidates for natural antioxidants and anticancer.

Key words: Lespedeza, Antineoplastic agents, Anti-oxidants, Anti-infective agents