Abstract: OBJECTIVE: To investigate the effect of Sharbat-e-Deenar(SD) on acetaminophen(APAP)-induced hepatotoxicity in rat model.METHODS: Albino rats were treated with SD at the doses of 1, 2 and 4 mL/kg, p.o. against hepatotoxicity after APAP(2 g/kg, p.o. once only) intoxication.The blood, tissue biochemical parameters and histopathological observation were performed. The RESULTS: APAP exposure in rats significantly increased the level of biochemical parameters such as aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, serum alkaline phosphatase, bilirubin, urea and creatinine into blood circulation which were reversed towards normal by SD therapy at all doses. The tissue biochemical parameters such as lipid peroxidation, reduced glutathione, adenosine tri-phosphatase and glucose-6-phosphatase were significantly restored after SD treatment against hepatotoxity. Histological analysis confirmed that SD-treated rats significantly alleviated of liver damage and reduced lesions caused by APAP intoxication. The biochemical changes are in good correlation with the histopathological observations.CONCLUSION: On the basis of these results, it can be concluded that SD exerts hepatoprotective activity against APAP-induced liver injury.

Key words: Sharbat-e-Deenar, Acetaminophen, Hepatitis, toxic, Hepatoprotection