Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (03): 333-340.

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Neuroprotective effect of Naomaitong extract following focal cerebral ischemia induced by middle cerebral artery occlusion in rats

Yang Yongxia, Chen Xi, Wang Shumei, Wang Zhanhong, Li Jiansheng, Liang Shengwang   

  1. College of Basic courses, Guangdong Pharmaceutical University;Division of Education and Research, Shantou Central Hospital;College of Traditional Chinese Medicine,Guangdong Pharmaceutical University;College of Traditional Chinese Medicine, Guangdong Pharmaceutical University;Institute of Geriatrics, Henan College of Traditional Chinese Medicine;
  • Accepted:2017-06-15 Online:2017-06-15 Published:2022-04-04
  • Supported by:
    Supported by National Natural Science Foundation of China(Study on the Material Basis and the Ratio of the Effective Components of Naodesheng Based on the Combination of Fingerprint and Metabolic Network,No.81274059;Study on the Material Basis of Naomaitong in the Treatment of Ischemic Stroke Based on the in vivo Dynamic Effect and Bioinformatics,No.81274060;Study on the in vivo Process and Compatibility Rule of Naomaitong Based on the PK-PD of Effective Components and the Multiobjective Optimization,No.81473413)

Abstract: OBJECTIVE: To examine the neuroprotective effect of extract from Naomaitong following focal cerebral ischemia reperfusion induced by occlusion of middle cerebral artery(MCA), and to determine the biochemical alterations in urine using proton nuclear magnetic resonance spectroscopy and principal component analysis.METHODS: Wistar rats were randomly assigned tothree groups: sham-operated group, MCA focal cerebral ischemia reperfusion model group, and active extract of Naomaitong treatment group. The model was established by an improved MCA occlusion(MCAO) method. Sham-operated rats received the same surgical procedure, but without occlusion. The Naomaitong treatment group were treated with active extract from Naomaitong at a dose of3.0 g·kg-·1d-1. Brain tissues and urine samples were collected from all groups for histopathological assessment and proton nuclear magnetic resonance spectroscopy-based metabonomics, respectively.RESULTS: Hematoxylin-eosin and triphenyl tetrazolium chloride staining of brain tissues showed a significant decrease in cerebral infarction area in the Naomaitong group. In model rats, metabonomic analyses showed increased urinary levels of glutamate, taurine, trimetlylamine oxide, betaine, and glycine, and reduced levels of creatinine and creatine.Naomaitong regulated the metabolic changes by acting on multiple metabolic pathways, including glycine metabolism, glutaminolysis, transmethylation metabolism and creatinine metabolism.CONCLUSION: These data demonstrate that extract from Naomaitong is neuroprotective against focal cerebral ischemia induced by MCAO, and can alleviate biochemical changes in urinary metabolism. Metabonomics may be a useful approach for assessing the biochemical mechanisms underlying the neuroprotective actions of extract from Naomaitong.

Key words: Cerebrovascular circulation, Reperfusion, Middle cerebral artery, Principal component analysis, Metabonomics, Magnetic resonance spectroscopy, Naomaitong

Cite this article

Yang Yongxia, Chen Xi, Wang Shumei, Wang Zhanhong, Li Jiansheng, Liang Shengwang. Neuroprotective effect of Naomaitong extract following focal cerebral ischemia induced by middle cerebral artery occlusion in rats[J]. Journal of Traditional Chinese Medicine, 2017, 37(03): 333-340.