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Journal of Traditional Chinese Medicine ›› 2017, Vol. 37 ›› Issue (01): 116-123.

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Mechanism studies of Xinfeng capsule on improving cardiovascular function though toll-like receptor 4/nuclear factor kappa B pathway in a rat model of adjuvant arthritis

Wang Yuan, Liu Jian, Huang Chuanbing, Sun Yue, Zhang Wandong, Wan Lei, Zong Ruikai, Wang Yali   

  1. Department of Rheumatology, First Affiliated Hospital, Anhui University of Traditional Chinese Medicine;Graduate Faculty, Anhui University of Traditional Chinese Medicine;
  • Accepted:2017-02-15 Online:2017-02-15 Published:2022-04-04
  • Supported by:
    the National Natural Science Foundation of China:Research the Immune Mechanism of Xinfeng Capsule Treat the Heart Disease of AA Rats based on the mi R146a-TLR4/NF-κB Signal Pathway(No.81302967), The Key Subject Constructing Units by the State Administrative Bureau(No.[2009]30), Anhui Provincial Laboratory Construction Projects on Chinese Medicine Research and Development(No.[2008]150), Anhui Provincial Innovation Team in the Eleventh Five-Year Plan Period:Research and Development on Xin'an Medicine(2010TD005)

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Abstract: OBJECTIVE: To observe the impact of Xinfeng capsule(XFC) on cardiovascular function in adjuvant arthritis(AA) model rats and investigate the mechanism though toll-like receptor 4(TLR4)/nuclear factor kappa B(NF-κB) signaling pathway.METHODS: Seventy rats were randomly divided into seven groups: normal control(NC), model control(MC), tripterygium glycosides tablet(TPT),methotrexate(MTX), high, moderate and low dose XFC group. The administration began from day 19 after modeling for 30 day. Paw swelling, arthritic in-dex(AI), cardiac function indexes and myocardial pathological pattern were detected. The expression of TLR4, myeloid differentiation factor(My D) 88, interleukin-1 receptor-associated kinase(IRAK) 1, tumor necrosis factor receptor associated factor(TRAF) 6, NF-κB, tumor necrosis factor-alpha(TNF-α) proteins in myocardial tissue were determined by western blot method.RESULTS: Paw swelling and AI in MC group increased in MC group(P < 0.01), and decreased in high and moderate dose XFC groups(P < 0.01 or P > 0.05). Left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),heart rate(HR) were elevated in MC group(P < 0.01). LVSP in high dose XFC group were reduced more than other treatment groups(P < 0.01). The improvements on LVEDP, dp/dt-max were superior to MTX and low dose XFC group, and the improvement on CI was better than low dose XFC group(P < 0.05 or P < 0.01). Myocardial fibers arranged irregular in MC group with intracellular edema and mitochondria damage. The modifications on myocardial structural were shown in each treatment group, but more prominent in TPT, high and moderate dose XFC group. The proteins of TLR4, My D88, IRAK1, TRAF6, NF-κB, TNF-αwere highly expressed in MC group, and those proteins declined in high and moderate dose XFC group(P < 0.05 or P < 0.01). High dose XFC group was superior to MTX and low dose XFC group on reducing TLR4, NF-κB, TNF-α(P < 0.05).CONCLUSION: XFC can not only inhibit the excessive activation of TLR4/NF-κB signaling pathway and the increased inflammatory mediators, but also reduce the damage of myocardial tissue and cells.

Key words: Arthritis, experimental, Cardiovascular diseases, Toll-like receptor 4, NF-kappa B, Signal transduction, Xinfeng capsule

Cite this article

Wang Yuan, Liu Jian, Huang Chuanbing, Sun Yue, Zhang Wandong, Wan Lei, Zong Ruikai, Wang Yali. Mechanism studies of Xinfeng capsule on improving cardiovascular function though toll-like receptor 4/nuclear factor kappa B pathway in a rat model of adjuvant arthritis[J]. Journal of Traditional Chinese Medicine, 2017, 37(01): 116-123.