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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (6): 928-937.DOI: 10.19852/j.cnki.jtcm.2020.06.005

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Lichong decoction inhibits micro-angiogenesis by reducing the expressions of hypoxia inducible factor-1α and vascular endothelial growth factor in hysteromyoma mouse model

Wang Wenna, Zhang Wufang, Li Donghua, Qian Ruiya, Zhu Lijuan, Liu Yu, Chen Chao   

  1. School of Traditional Chinese Medicine,Capital Medical University;Gynecology department, The fourth ward of Beijing Obstetrics and Gynecology Hospital, Capital Medical University Beijing Maternaland Child;
  • Online:2020-12-25 Published:2020-12-25
  • Supported by:
    Supported by the National Natural Science Foundation of China (to Study the Effect and Molecular Mechanism of Lichong Decoction in Inhibiting Immune Escape of Hysteromyoma Through IDO Mediated Immune Editing Pathway,No. 81774072, Based on Angiogenesis Microenvironment to Explore the Mechanism of Lichong Decoction in Inhibiting Hysteromyoma by Regulating Ang/Tie-2 Signal Transduction Pathway,No. 81373812, Objective to Explore the Regulatory Mechanism of Extracellular Matrix Metabolism of Fuzheng Quyu Traditional Chinese Medicine on the Inhibition of Hysteromyoma,No. 81073096), Beijing Municipal Natural Science Foundation (Effect of Lichong Decoction on Balance Drift of Angiogenesis/Inhibitor of Hysteromyoma Based on Lnc RNA Signal Pathway,7202015), Inheritance and Innovation of Traditional Chinese Medicine"Hundred,Thousand and Ten Thousand"Talent Project (Qihuang Project) the Fourth Batch of National Research and Training Project of Chinese Medicine Talents (TCM Education And Development 2017 No. 24)

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Abstract: OBJECTIVE: To investigate the efficacy of Lichong decoction(LD) from Traditional Chinese Medicine,on micro-angiogenesis in a mouse model of hysteromyoma.METHODS: A mouse model of hysteromyoma was developed by orthotopic intrauterine injection of primary human myoma cells isolated from patients from the Beijing Obstetrics and Gynecology Hospital into CB-17 Scid mice. Mice were administered high-dose LD, low-dose LD, mifepristone or water(control) daily by gavage for 4 weeks. Uterine diameter and coefficient(uterine weight/body weight)were measured. Uterine morphology was assessed by light microscopy(hematoxylin and eosin) and transmission electron microscopy. Serum levels of estradiol, progesterone, follicle-stimulating hormone and luteinizing hormone(LH) were measured by enzyme-linked immunosorbent assay.Uterine protein expression of hypoxia inducible factor(HIF)-1α, CD31 and proliferating cell nuclear antigen(PCNA) was detected by immunohistochemistry. VEGF and HIF-1α m RNAs were quantified by RT-PCR.RESULTS: High-dose LD, low-dose LD and mifepristone reduced uterine diameter and coefficient, and attenuated the morphologic abnormalities associated with hysteromyoma. High-dose LD, low-dose LD and mifepristone inhibited hysteromyoma-induced micro-angiogenesis, as evidenced by a decrease in the number of new microvessels co-immunostaining for CD31 and PCNA(P < 0.01).High-dose LD and mifepristone lowered serum levels of estradiol, progesterone and LH(P < 0.05).High-dose LD, low-dose LD and mifepristone down-regulated HIF-1α m RNA and protein expressions and VEGF m RNA expression(P < 0.01).CONCLUSION: The inhibition of hysteromyoma by LD may involve reductions in HIF-1α and VEGF expression and suppression of micro-angiogenesis.

Key words: Leiomyoma, Neovascularization,pathologic, Hypoxia-inducible factor-1α, Vascular endothelial growth factors, Lichong decoction

Cite this article

Wang Wenna, Zhang Wufang, Li Donghua, Qian Ruiya, Zhu Lijuan, Liu Yu, Chen Chao. Lichong decoction inhibits micro-angiogenesis by reducing the expressions of hypoxia inducible factor-1α and vascular endothelial growth factor in hysteromyoma mouse model[J]. Journal of Traditional Chinese Medicine, 2020, 40(6): 928-937.