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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (4): 562-570.DOI: 10.19852/j.cnki.jtcm.2020.04.005

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Effect of Baizhu(Rhizoma Atractylodis Macrocephalae) extract on intestinal absorption of brucine and strychnine in vitro and in situ

Lü Dan, Jiang Qieying, Zhang Jing, Zeng Ronggui, Liao Zhenggen, Liang Xinli   

  1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine of Ministry of Education, Jiangxi University of Traditional Chinese Medicine;Jiangsu College of Nursing;Key Laboratory of Modern Preparation of Traditional Chi-nese Medicine of Ministry of Education, Jiangxi University ofTraditional Chinese Medicine;
  • Online:2020-08-15 Published:2020-08-15
  • Supported by:
    the National Natural Science Foundation of China (No. 81660757 and No. 81303237), the Academic and Technological Foregoer Funds of Jiangxi Province,China(No. 20162BCB22015), the Project on Cultivation of Medical Elite (Gan Po Ying Cai 555)(2013296), the Youth Science Funds of Jiangxi Province,China (No. 20153BCB23019), the National Natural Science Foundation of Jiangxi Province(No. 20161ACB21020), the Natural Science Research Project of Huaian (No. HAB201716)

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Abstract: OBJECTIVE: To investigate the antagonistic effect of the extract of Baizhu(Rhizoma Atractylodis Macrocephalae)(RAM) on the intestinal absorption of brucine and strychnine in Strychnos nux-vomica(NUX) and propose the mechanism of these effects.METHODS: The apparent permeability value(Papp)and absorption rate constant(Ka) were chosen as indices. The everted intestinal sac model and in situ single-pass intestinal perfusion model were used to study the effects of the RAM extract on the absorption of brucine and strychnine. To confirm the results, the brucine and strychnine concentrations in hepatic portal venous blood were determined.Western blotting was used to study P-glycoprotein(P-gp) expression in the Caco-2 cell line.RESULTS: Pappand Kaof brucine and strychnine were significantly increased in the presence of a P-gp inhibitor, but no significant increase was noted in the presence of a tight junction regulator. The RAM extract inhibited the absorption of brucine and strychnine and enhanced P-gp expression.CONCLUSION: The primary absorption mechanism for brucine and strychnine is passive transport,which is affected by P-gp.

Key words: Strychnos nux-vomica, Brucine, Strychnine, ATP binding cassette transporter,subfamily B,member 1, Baizhu(Rhizoma Atractylodis Macrocephalae)

Cite this article

Lü Dan, Jiang Qieying, Zhang Jing, Zeng Ronggui, Liao Zhenggen, Liang Xinli. Effect of Baizhu(Rhizoma Atractylodis Macrocephalae) extract on intestinal absorption of brucine and strychnine in vitro and in situ[J]. Journal of Traditional Chinese Medicine, 2020, 40(4): 562-570.