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Journal of Traditional Chinese Medicine ›› 2020, Vol. 40 ›› Issue (3): 407-413.DOI: 10.19852/j.cnki.jtcm.2020.03.008

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Electroacupuncture relieves irritable bowel syndrome by increasing expression of nucleotide-binding oligomerization domain protein-like receptor family pyrin domain containing 6 in water-avoidance stress mice

Song Yafang, Geng Hao, Chen Lu, Wu Xiaoliang, Yuan Mengqian, Xu Wanli, Guo Jing, Sun Jianhua, Pei Lixia   

  1. Department of acupuncture, The Affiliated Hospital of Nanjing University of Chinese Meidicne;Department of the First Clinical Medical College, Nanjing University of Chinese Medicine;
  • Online:2020-06-15 Published:2020-06-15
  • Supported by:
    Supported by the National Natural Science Fund (No.81473748,81804193), the Youth Natural Foundation of the Science and Technology Department of Jiangsu Province(No. BK20161083), a Grant from Clinical Medicine Center of Digestive Diseases of Traditional Chinese Medicine in Jiangsu (No. BL2014100-4), the Leading Talents Project from Jiangsu Provincial Administration of TCM (SLJ0206), the Science and Technology Project of Jiangsu Provincial Administration of TCM (No. JD201506), the peak talent project of Jiangsu Province Hospital of TCM (No. y2018rc05)

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Abstract: OBJECTIVE: To investigate the effect of electroacupuncture(EA) on irritable bowel syndrome(IBS) in mice through regulating nucleotide-binding oligomerization domain protein-like receptor family pyrin domain containing 6(NLRP6).METHODS: Water-avoidance stress(WAS) mice model was used to investigate the effects and the mechanism of EA. Abdominal withdrawal reflex test, open field test, and intestinal motility test were used to evaluate visceral sensitivity, anxiety,and intestinal motility in mice. The expressions of NLRP6, Mucin-2(MUC2) and E-cadherin were determined using immunofluorescence and Western blotting assays.RESULTS: EA significantly upregulated the expression of NLRP6 in the intestine of mice. Moreover, EA increased the expressions of MUC2 and E-cadherin in WAS mice.CONCLUSION: Our study found that the relief of IBS symptoms by EA may involve the increase in the expression of NLRP6 in WAS mice.

Key words: Electroacupuncture, Nod signaling adaptor proteins, Irritable bowel syndrome

Cite this article

Song Yafang, Geng Hao, Chen Lu, Wu Xiaoliang, Yuan Mengqian, Xu Wanli, Guo Jing, Sun Jianhua, Pei Lixia. Electroacupuncture relieves irritable bowel syndrome by increasing expression of nucleotide-binding oligomerization domain protein-like receptor family pyrin domain containing 6 in water-avoidance stress mice[J]. Journal of Traditional Chinese Medicine, 2020, 40(3): 407-413.