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Journal of Traditional Chinese Medicine ›› 2021, Vol. 41 ›› Issue (1): 98-106.DOI: 10.19852/j.cnki.jtcm.2021.01.012

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Regulative effect of Taohong Siwu decoction(桃红四物汤) on extracellular matrix of endometrium in drug-induced abortion

WANG Mengmeng, GUO Dongdong, HAN Lan, PENG Daiyin, CHEN Weidong, ZHANG Yanyan   

  1. Anhui Province Key Laboratory of Chinese Medicinal Formula, School of Pharmacy, Anhui University of Chinese Medicine;Key Laboratory of Xin'an Medicine, Ministry of Education, School of Pharmacy, Anhui University of Chinese Medicine;
  • Online:2021-02-03 Published:2021-06-03
  • Supported by:
    Supported by Natural Science Foundation-funded Projects: Regulation Effect of Taohong-Siwu Decoction on Alpha-granule Release of Platelet in Postpartum Blood Stasis, its Active Compounds and Action Pathway (No. 81473387), Mechanism Research of Taohong-Siwu Decoction in Regulating Brain Microvascular Angiogenesis of MACO Rats Based on Platelet Microparticles as a Plaletet-Brain Microvascular Cell Messenger (No. 81503291), Natural Science Foundation Projects of Anhui Colleges and Universities: Based on the Crosstalk of NLRP3-Caspase-1 and VEGF-Notch to Explore the Effect of THSWD Regulation of "Dispelling Stasis to Promote Regeneration" by the Interaction Between Pyroptosis and Angiogenesis After Cerebral Ischemia and Reperfusion (No. KJ2019A0467)

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Abstract: OBJECTIVE: To explore the effects of Taohong Siwu decoction(桃红四物汤, THSWD) on the extracellular matrix of endometrium in rats following drug-induced abortion.METHODS: Thirty-six pregnant female rats were administered mifepristone and misoprostol to induce abortion, and amounts of uterine bleeding were recorded. Pathological damage and collagen accumulation were detected by hematoxylin-eosin staining and Masson's trichrome staining in uterus, respectively. Myeloperoxidase was evaluated by immunohistochemistry.The expression levels of fibronectin, laminin, matrix metalloproteinase 9(MMP-9), and tissue inhibitor of metalloproteinase 1(TIMP-1) were quantified using western blotting.RESULTS: THSWD could promote endometrial protection in rats following drug-induced abortion.The contents of cellulose and myeloperoxidase were significantly decreased in uterine tissue of THSWD-treated groups. Moreover, THSWD significantly decreased the expression levels of fibronectin, laminin, and TIMP-1. THSWD also significantly increased MMP-9 expression and the MMP-9/TIMP-1 ratio.CONCLUSION: THSWD plays a critical role in endometrial protection by reducing extracellular matrix deposition and uterine fibrosis. These effects may have been achieved by increasing MMP-9, reducing TIMP-1, and/or altering the ratio of MMP-9/TIMP-1.

Key words: Abortion,induced, extracellular matrix, endometrium, matrix metalloproteinase 9, tissue inhibitor of metalloproteinase-1, Taohong Siwu decoction

Cite this article

WANG Mengmeng, GUO Dongdong, HAN Lan, PENG Daiyin, CHEN Weidong, ZHANG Yanyan. Regulative effect of Taohong Siwu decoction(桃红四物汤) on extracellular matrix of endometrium in drug-induced abortion[J]. Journal of Traditional Chinese Medicine, 2021, 41(1): 98-106.